CLK1 Antibody
(Synonyms: CLK, CLK1, Dual specificity protein kinase CLK1, CDC-like kinase 1)CLK1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CLK1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
CLK1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to CLK1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 57 kDa
Synthetic peptide corresponding to the center region of human CLK1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CLK1 is a Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells[1][2].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Endothelial cells. -
Isoforms & Post-Translational Modification
CLK1 has 3 isoforms, P49759-1: amino acid length is 484, molecular weight is 57291 Da (predicted); P49759-2: amino acid length is 136, molecular weight is 16570 Da (predicted); P49759-3: amino acid length is 526, molecular weight is 61757 Da (predicted).
Autophosphorylates on all three types of residues. -
Subunit
Interacts with PPIG and UBL5
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SwissProt ID
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Synonyms
CLK, CLK1, Dual specificity protein kinase CLK1, CDC-like kinase 1
Documentation
References
[1]. Moeslein FM, et al. The CLK family kinases, CLK1 and CLK2, phosphorylate and activate the tyrosine phosphatase, PTP-1B. J Biol Chem. 1999 Sep 17;274(38):26697-704. [Content Brief]
[2]. Eisenreich A, et al. Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in human endothelial cells. Circ Res. 2009 Mar 13;104(5):589-99. [Content Brief]
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