FGF-2 Antibody (YA4852)
(Synonyms: FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2)FGF-2 Antibody (YA4852) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FGF-2.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, IP, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS(7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:50-100 | 1:10000 |
Product Details
FGF-2 Antibody (YA4852) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FGF-2.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 17-25 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 31 kDa
Purified recombinant fragment of Human FGF-2.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS(7.4) containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
FGF-2 acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation[1][2][3][4][5][6][7].
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Subcellular Localization
Secreted; Nucleus
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Expression
Tissue_specificity:It is expressed in granulosa cells and cumulus cells. It is expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
Isoforms & Post-Translational Modification
P09038 has 4 isomers: P09038-4: 30770 Da (predicted); P09038-1: 22623 Da (predicted); P09038-2: 17254 Da (predicted); P09038-3: 21203 Da (predicted).
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion;Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing -
Subunit
Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP3 (PubMed:18669637). Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling (PubMed:28302677). Interacts with SNORC (via the extracellular domain) (By similarity). Interacts with glypican GPC3 (By similarity)
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SwissProt ID
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Synonyms
FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
Documentation
References
[1]. Ornitz DM, et al. Receptor specificity of the fibroblast growth factor family. J Biol Chem. 1996 Jun 21;271(25):15292-7. [Content Brief]
[2]. Mori S, et al. The integrin-binding defective FGF2 mutants potently suppress FGF2 signalling and angiogenesis. Biosci Rep. 2017 Apr 28;37(2):. [Content Brief]
[3]. Shimoyama Y, et al. Characterization of high-molecular-mass forms of basic fibroblast growth factor produced by hepatocellular carcinoma cells: possible involvement of basic fibroblast growth factor in hepatocarcinogenesis. Jpn J Cancer Res. 1991 Nov;82(11):1263-70. [Content Brief]
[4]. Gautschi P, et al. Partial molecular characterization of endothelial cell mitogens from human brain: acidic and basic fibroblast growth factors. FEBS Lett. 1986 Aug 18;204(2):203-7. [Content Brief]
[5]. Gimenez-Gallego G, et al. Human brain-derived acidic and basic fibroblast growth factors: amino terminal sequences and specific mitogenic activities. Biochem Biophys Res Commun. 1986 Mar 13;135(2):541-8. [Content Brief]
[6]. Mori S, et al. A dominant-negative FGF1 mutant (the R50E mutant) suppresses tumorigenesis and angiogenesis. PLoS One. 2013;8(2):e57927. [Content Brief]
[7]. Zhao G, et al. Negative regulation of lens fiber cell differentiation by RTK antagonists Spry and Spred. Exp Eye Res. 2018 May;170:148-159. [Content Brief]
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