SOX2 Antibody (YA4045)(PBS only)

Cat. No.: HY-P84348A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

SOX2 Antibody (YA4045) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOX2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SOX2 Antibody (YA4045) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOX2.

Host

Mouse

Molecular Weight

Predicted band size: 34 kDa;

Observed band size: 34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit, Monkey

SwissProt ID

P48431

Gene ID

6657 [NCBI]

Immunogen

Purified recombinant fragment of human SOX2 aa 1-170.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity)

Subcellular Localization：Nucleus speckle; Cytoplasm; Nucleus

Subunit：Interacts with ZSCAN10 (By similarity). Interacts with SOX3 and FGFR1 (By similarity). Interacts with GLIS1 (PubMed:21654807). Interacts with POU5F1; binds synergistically with POU5F1 to DNA (By similarity). Interacts with DDX56 (By similarity). Interacts with L3MBTL3 and DCAF5; the interaction requires methylation at Lys-42 and is necessary to target SOX2 for ubiquitination by the CRL4-DCAF5 E3 ubiquitin ligase complex (PubMed:30442713). Interacts with RCOR1/CoREST (By similarity) (PubMed:30442713). Interacts with PHF20L1; the interaction requires methylation at Lys-42 and Lys-117 and protects SOX2 from degradation (PubMed:29358331). Interacts with TRIM26; this interaction prevents ubiquitination by WWP2 (PubMed:34732716)

Synonyms

ANOP3; MCOPS3

Documentation

User Guide for Antibodies (1077 KB)