Visfatin Antibody (YA5011)(PBS only)

Cat. No.: HY-P85319A: Data Sheet User Guide for Antibodies
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Visfatin Antibody (YA5011) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Visfatin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Visfatin Antibody (YA5011) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Visfatin.

Host

Mouse

Molecular Weight

Predicted band size: 56 kDa;

Species Reactivity

Human, Rat, Mouse, Monkey, Rabbit

SwissProt ID

P43490

Gene ID

10135 [NCBI]

Immunogen

Purified recombinant fragment of PBEF1 (aa338-479) expressed in E. Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity)

Subcellular Localization：Nucleus; Cytoplasm; Secreted

Expression：
Tissue_specificity:It is abundant in bone marrow, liver tissue, and muscles. It is also found in heart, placenta, lung, and kidney tissues.

Subunit：Homodimer

Synonyms

NAMPT; PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

Documentation

User Guide for Antibodies (1077 KB)