2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies Modification Specific Antibodies
  5. Phospho-Tau (Ser396) Antibody (YA140)

Phospho-Tau (Ser396) Antibody (YA140)

Cat. No.: HY-P80480
COA
SDS
User Guide for Antibodies Technical Support

Phospho-Tau (Ser396) Antibody (YA140) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (Ser396).

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

Description

Phospho-Tau (Ser396) Antibody (YA140) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (Ser396).
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 79 kDa;
Observed band size: 70/130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat, Monkey, Pig
SwissProt ID
Gene ID
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser396 of Human Tau.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:500
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:200-1:500
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:100-1:500
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
ALL ICC IHC-P

  • Immunocytochemistry analysis of Hela cells labeling Phospho-Tau (Ser396) with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Tau (Ser396) Antibody (HY-P80480)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling Phospho-Tau (Ser396) with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-Tau (Ser396) Antibody (HY-P80480) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background
Function：Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization
Subcellular Localization：Cytoplasm, cytosol; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cytoskeleton; Cell projection, axon; Cell projection, dendrite; Secreted
Expression：
Tissue_specificity:It is expressed in neurons. The PNS-tau subtype is expressed in the peripheral nervous system, while other subtypes are expressed in the central nervous system.
Subunit：Interacts with MARK1, MARK2, MARK3 and MARK4 (PubMed:23666762). Interacts with PSMC2 through SQSTM1 (By similarity). Interacts with SQSTM1 when polyubiquitinated (PubMed:15953362). Interacts with FKBP4 (By similarity). Binds to CSNK1D (PubMed:14761950). Interacts with SGK1 (PubMed:16982696). Interacts with EPM2A; the interaction dephosphorylates MAPT at Ser-396 (PubMed:19542233). Interacts with PIN1 (PubMed:11313338). Interacts with LRRK2 (PubMed:26014385). Interacts with LRP1, leading to endocytosis; this interaction is reduced in the presence of LRPAP1/RAP (PubMed:32296178)
RRID
Database

Entrez Gene: 4137 Human ; 17762 Mouse ;

SwissProt: P10636 Human ; P10637 Mouse ; P19332 Rat

OMIM: 600274 Human

Research Field

Neuroscience
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-Tau (Ser396) Antibody (YA140) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Phospho-Tau (Ser396) Antibody (YA140)
Cat. No.:
HY-P80480
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.