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ABCE1 Antibody (YA1699)

(Synonyms: ABC38; ABCE1; HuHP68; OABP; RLI; RNASEL1; RNASELI; RNS4I)
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Based on 1 Customer Validation

ABCE1 Antibody (YA1699) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCE1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ABCE1 Antibody (YA1699)
  • ABCE1 Antibody (YA1699) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

ABCE1 Antibody (YA1699) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ABCE1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ABCE1 aa586-599.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104075

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ABCE1 Antibody (YA1699)
    Western blot analysis of extracts from K562(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ABCE1 Antibody (HY-P81954) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ABCE1 is a Nucleoside-triphosphatase (NTPase) involved in ribosome recycling by mediating ribosome disassembly. Able to hydrolyze ATP, GTP, UTP and CTP. Splits ribosomes into free 60S subunits and tRNA- and mRNA-bound 40S subunits. Acts either after canonical termination facilitated by release factors (ETF1/eRF1) or after recognition of stalled and vacant ribosomes by mRNA surveillance factors (PELO/Pelota). Involved in the No-Go Decay (NGD) pathway: recruited to stalled ribosomes by the Pelota-HBS1L complex, and drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. Also plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, ubiquitinated by CNOT4 upon mitochondria damage; this modification generates polyubiquitin signals that recruit autophagy receptors to the mitochondrial outer membrane and initiate mitophagy. RNASEL-specific protein inhibitor which antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) to RNASEL. Negative regulator of the anti-viral effect of the interferon-regulated 2-5A/RNASEL pathway; (Microbial infection) May act as a chaperone for post-translational events during HIV-1 capsid assembly; (Microbial infection) Plays a role in the down-regulation of the 2-5A/RNASEL pathway during encephalomyocarditis virus (EMCV) and HIV-1 infections[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm; Mitochondrion

  • Expression


    Induction:Activated by encephalomyocarditis virus (EMCV) and HIV-1

  • Subunit

    (Microbial infection) Interacts with Chandipura virus matrix protein

  • SwissProt ID

    P61221

  • Gene ID
    6059 [NCBI]
  • Synonyms

    ABC38; ABCE1; HuHP68; OABP; RLI; RNASEL1; RNASELI; RNS4I

  • Research Field

    Signal Transduction

ABCE1 Antibody (YA1699) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ABCE1 Antibody (HY-P81954) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ABCE1 Antibody (YA1699) Product Image
    1/16
  • Experimental Validation Results for ABCE1 Antibody (YA1699)
  • ABCE1 Antibody (YA1699) Product Image
  • Experimental Validation Results for ABCE1 Antibody (YA1699)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ABCE1 Antibody (HY-P81954) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ABCE1 Antibody (YA1699)
  • ABCE1 Antibody (YA1699) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using ABCE1 Antibody (HY-P81954) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ABCE1 Antibody (YA1699) Product Image WB