GAL4 Antibody (YA3377)

Cat. No.: HY-P83632: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

GAL4 Antibody (YA3377) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAL4.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈	재고	수량
무료 샘플	Apply now
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

GAL4 Antibody (YA3377) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
제품 설명

제품 설명

GAL4 Antibody (YA3377) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAL4.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P56470

Gene ID

3960 [NCBI]

Immunogen

A synthesized peptide derived from human GAL4 aa1-55/323.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions

Subunit：Monomer

RRID

AB_3105739

Database

Entrez Gene: 3960 Human ; 25474 Rat

SwissProt: P56470 Human ; P56470 Human ; P38552 Rat

OMIM: 602518 Human

Research Field

Tags & Cell Markers

Synonyms

L36LBP; Lactose binding lectin 4; Lectin galactoside binding soluble 4; LGALS4

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

GAL4 Antibody (YA3377) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

최근 본 상품:

온라인 문의

Your information is safe with us. * Required Fields.

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
상품명			Requested Quantity *
고객명 *			호칭
메일주소 *			전화번호 *
Department			회사명 *
City			도
Country or Region *
비고

대량구매 문의

Inquiry Information

상품명:: GAL4 Antibody (YA3377)
Cat. No.:: HY-P83632
수량:
MCE Japan Authorized Agent:

1/16

MedchemExpress Validation
Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
1/16

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

MedChemExpress Validation
Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

상품명			Lot #
고객명 *			Country or Region *
메일주소 *
전화번호 *			회사명 *
비고

MedChemExpress: Contact Us; About Us; Headquarters; Distributors; Statement on False Report; Careers

고객지원: Technical Support; 서비스; Ordering Information; 편리한 반품; 배송요금 및 정책; Intellectual Property Promise

도구: 무료 샘플 정책; Resources; 몰농도 계산기; 농도 희석 계산기; Terms & Conditions; Reconstitution Calculator; Specific Activity Calculator; DNA/RNA sequence conversion tools

E-newsletter 구독

Name
이메일 *

	Sorry, but the email address you supplied was invalid.

제품은 연구용 화학 시약이며 사람에게 사용할 수 없습니다. 환자에게는 판매하지 않습니다.

Join with us

GAL4 Antibody (YA3377)

GAL4 Antibody (YA3377) Featured Recommendations:

Product Detail

Verification Image

Background

제품 설명

GAL4 Antibody (YA3377) Related Classifications

최근 본 상품:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report