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GAL4 Antibody (YA3377)

(Synonyms: L36LBP; Lactose binding lectin 4; Lectin galactoside binding soluble 4; LGALS4)
Customer Review

Based on 1 Customer Validation

GAL4 Antibody (YA3377) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAL4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • GAL4 Antibody (YA3377) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

GAL4 Antibody (YA3377) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GAL4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GAL4 aa1-55/323.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105739

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GAL4 Antibody (YA3377)
    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GAL4 Antibody (YA3377)
    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAL4 Antibody (YA3377)
    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    GAL4 is a Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions

  • Subunit

    Monomer

  • SwissProt ID

    P56470

  • Gene ID
    3960 [NCBI]
  • Synonyms

    L36LBP; Lactose binding lectin 4; Lectin galactoside binding soluble 4; LGALS4

  • Research Field

    Tags & Cell Markers

GAL4 Antibody (YA3377) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GAL4 Antibody (YA3377) Product Image
    1/16
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • GAL4 Antibody (YA3377) Product Image
  • Experimental Validation Results for GAL4 Antibody (YA3377)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GAL4 Antibody (YA3377)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAL4 Antibody (YA3377)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • Experimental Validation Results for GAL4 Antibody (YA3377)
  • GAL4 Antibody (YA3377) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HT-29 (lane 1, 15 μg), Rat colon (lane 2, 15 μg), and Mouse colon (lane 3, 15 μg) using GAL4 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GAL4 Antibody (YA3377) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GAL4 Antibody (YA3377) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue using GAL4 Antibody (YA3377). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83632, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • GAL4 Antibody (YA3377) Product Image WB
  • GAL4 Antibody (YA3377) Product Image IHC-P
  • GAL4 Antibody (YA3377) Product Image IHC-P