Wilms Tumor Protein Antibody (YA2277)

Cat. No.: HY-P82532: Data Sheet COA
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User Guide for Antibodies
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Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

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50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
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Wilms Tumor Protein Antibody (YA2277) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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References

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Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 49 kDa;

Observed band size: 55 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P19544

Gene ID

7490 [NCBI]

Immunogen

A synthesized peptide derived from human Wilms Tumor Protein

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis was performed on extracts from K562 (lane 1, 20 μg) using Wilms Tumor Protein Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

Function：Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921)

Subcellular Localization：Nucleus; Nucleus, nucleolus; Cytoplasm; Nucleus speckle; Nucleus, nucleoplasm

Expression：
Tissue_specificity:It is expressed in the kidneys and some hematopoietic cells.

Isoforms & Post-Translational Modification：P19544 has 8 isomers: P19544-1: 49188 Da (predicted); P19544-2: 47195 Da (predicted); P19544-3: 47511 Da (predicted); P19544-4: 48872 Da (predicted); P19544-6: 34447 Da (predicted); P19544-7: 56883 Da (predicted); P19544-8: 55206 Da (predicted); P19544-9: 33086 Da (predicted).

Subunit：Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with HNRNPU via the zinc-finger region. Interacts with U2AF2. Interacts with CITED2 (By similarity). Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP and SRY. Interacts with AMER1. Interacts with RBM4

RRID

AB_3104637

Database

Entrez Gene: 7490 Human ;

SwissProt: P19544 Human ; P22561 Mouse ;

OMIM: 136680 Human

Research Field

Tags & Cell Markers

Synonyms

GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; WIT 2 antibody; WT 1 antibody; FWT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms Tumor antibody; Wilms tumor protein antibody; Wilms' tumor gene antibody; WIT2 antibody; WT antibody; WT1 antibody; WT1_HUMAN antibody; WT33 antibody

각종 서류

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hela-cell.html [Content Brief]

Wilms Tumor Protein Antibody (YA2277) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Wilms Tumor Protein Antibody (YA2277)

Wilms Tumor Protein Antibody (YA2277) Featured Recommendations:

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Background

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Wilms Tumor Protein Antibody (YA2277) Related Classifications

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