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Wilms Tumor Protein Antibody (YA2277)

(Synonyms: GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; WIT 2 antibody; WT 1 antibody; FWT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms Tumor antibody; Wilms tumor protein antibody; Wilms' tumor gene antibody; WIT2 antibody; WT antibody; WT1 antibody; WT1_HUMAN antibody; WT33 antibody)
Customer Review

Based on 1 Customer Validation

Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Wilms Tumor Protein Antibody (YA2277) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

Wilms Tumor Protein Antibody (YA2277) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wilms Tumor Protein.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 49 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Wilms Tumor Protein

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104637

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    Western blot analysis was performed on extracts from K562 (lane 1, 20 μg) using Wilms Tumor Protein Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    Flow cytometric analysis of 1X106 K562 cells labeling Wilms Tumor Protein Antibody (HY-P82532, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Wilms Tumor Protein transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus; Nucleus, nucleolus; Cytoplasm; Nucleus speckle; Nucleus, nucleoplasm

  • Expression


    Tissue_specificity:It is expressed in the kidneys and some hematopoietic cells.

  • Isoforms & Post-Translational Modification

    P19544 has 8 isomers: P19544-1: 49188 Da (predicted); P19544-2: 47195 Da (predicted); P19544-3: 47511 Da (predicted); P19544-4: 48872 Da (predicted); P19544-6: 34447 Da (predicted); P19544-7: 56883 Da (predicted); P19544-8: 55206 Da (predicted); P19544-9: 33086 Da (predicted).

  • Subunit

    Homodimer. Interacts with WTIP. Interacts with actively translating polysomes. Detected in nuclear ribonucleoprotein (mRNP) particles. Interacts with HNRNPU via the zinc-finger region. Interacts with U2AF2. Interacts with CITED2 (By similarity). Interacts with ZNF224 via the zinc-finger region. Interacts with WTAP and SRY. Interacts with AMER1. Interacts with RBM4

  • SwissProt ID

    P19544

  • Gene ID
    7490 [NCBI]
  • Synonyms

    GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1; WIT 2 antibody; WT 1 antibody; FWT1 antibody; GUD antibody; NPHS4 antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms Tumor antibody; Wilms tumor protein antibody; Wilms' tumor gene antibody; WIT2 antibody; WT antibody; WT1 antibody; WT1_HUMAN antibody; WT33 antibody

  • Research Field

    Tags & Cell Markers

Wilms Tumor Protein Antibody (YA2277) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 20 μg) using Wilms Tumor Protein Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling Wilms Tumor Protein Antibody (HY-P82532, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Wilms Tumor Protein Antibody (YA2277) Product Image
    1/16
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Wilms Tumor Protein Antibody (YA2277) Product Image
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from K562 (lane 1, 20 μg) using Wilms Tumor Protein Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling Wilms Tumor Protein Antibody (HY-P82532, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Experimental Validation Results for Wilms Tumor Protein Antibody (YA2277)
  • Wilms Tumor Protein Antibody (YA2277) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from K562 (lane 1, 20 μg) using Wilms Tumor Protein Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Wilms Tumor Protein Antibody (YA2277) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adenolymphoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Wilms Tumor Protein Antibody (YA2277) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Hepatocellular Carcinoma tissue using Wilms Tumor Protein antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82532, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Wilms Tumor Protein Antibody (YA2277) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling Wilms Tumor Protein Antibody (HY-P82532, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Wilms Tumor Protein Antibody (YA2277) Product Image WB
  • Wilms Tumor Protein Antibody (YA2277) Product Image IHC-P
  • Wilms Tumor Protein Antibody (YA2277) Product Image IHC-P
  • Wilms Tumor Protein Antibody (YA2277) Product Image FC