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Glucosidase 2 subunit beta Antibody (YA409)

(Synonyms: G19P1, PRKCSH, Glucosidase 2 subunit beta, 80K-H protein, Glucosidase II subunit beta, Protein kinase C substrate 60.1 kDa protein heavy chain, PKCSH)
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Based on 1 Customer Validation

Glucosidase 2 subunit beta Antibody (YA409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucosidase 2 subunit beta.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Glucosidase 2 subunit beta Antibody (YA409) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:50-1:200

Product Details

Description

Glucosidase 2 subunit beta Antibody (YA409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucosidase 2 subunit beta.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Species Reactivity Database

Entrez Gene: 5589 Human

SwissProt: P14314 Human

Immunogen

Synthetic peptide corresponding to Human Glucosidase 2 subunit beta.AA range:479-528.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102489

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
    Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg), K562(lane 4(20μg) and MCF-7(lane 5(20μg) ) using Glucosidase 2 subunit beta(HY-P80405) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80405, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), MCF-7 (lane 4), 293T (lane 5), A549 (lane 6) and HT-1080 (lane 7) using Glucosidase 2 subunit beta antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Glucosidase 2 subunit beta is a Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia[1].

  • Subcellular Localization

    Endoplasmic reticulum

  • Isoforms & Post-Translational Modification

    P14314 has 2 isomers: P14314-1: 59425 Da (predicted); P14314-2: 59178 Da (predicted).

  • Subunit

    Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH) (PubMed:10929008). Binds glycosylated PTPRC (By similarity)

  • SwissProt ID

    P14314

  • Gene ID
    5589 [NCBI]
  • Synonyms

    G19P1, PRKCSH, Glucosidase 2 subunit beta, 80K-H protein, Glucosidase II subunit beta, Protein kinase C substrate 60.1 kDa protein heavy chain, PKCSH

  • Research Field

    Tags & Cell Markers

[1]. Pelletier MF, et al. The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo. Glycobiology. 2000 Aug;10(8):815-27. [Content Brief]

Glucosidase 2 subunit beta Antibody (YA409) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg), K562(lane 4(20μg) and MCF-7(lane 5(20μg) ) using Glucosidase 2 subunit beta(HY-P80405) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80405, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), MCF-7 (lane 4), 293T (lane 5), A549 (lane 6) and HT-1080 (lane 7) using Glucosidase 2 subunit beta antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Glucosidase 2 subunit beta Antibody (YA409) Product Image
    1/16
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Glucosidase 2 subunit beta Antibody (YA409) Product Image
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg), K562(lane 4(20μg) and MCF-7(lane 5(20μg) ) using Glucosidase 2 subunit beta(HY-P80405) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80405, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), MCF-7 (lane 4), 293T (lane 5), A549 (lane 6) and HT-1080 (lane 7) using Glucosidase 2 subunit beta antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Experimental Validation Results for Glucosidase 2 subunit beta Antibody (YA409)
  • Glucosidase 2 subunit beta Antibody (YA409) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg), K562(lane 4(20μg) and MCF-7(lane 5(20μg) ) using Glucosidase 2 subunit beta(HY-P80405) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80405, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Glucosidase 2 subunit beta Antibody (YA409) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), K562 (lane 3), MCF-7 (lane 4), 293T (lane 5), A549 (lane 6) and HT-1080 (lane 7) using Glucosidase 2 subunit beta antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Glucosidase 2 subunit beta Antibody (YA409) Product Image WB
  • Glucosidase 2 subunit beta Antibody (YA409) Product Image WB