Glucosidase 2 subunit beta Antibody (YA409)

Cat. No.: HY-P80405: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Glucosidase 2 subunit beta Antibody (YA409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucosidase 2 subunit beta.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Glucosidase 2 subunit beta Antibody (YA409) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Glucosidase 2 subunit beta Antibody (YA409) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucosidase 2 subunit beta.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 59 kDa;

Observed band size: 90 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P14314

Gene ID

5589 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Glucosidase 2 subunit beta.AA range:479-528.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg), K562(lane 4(20μg) and MCF-7(lane 5(20μg) ) using Glucosidase 2 subunit beta(HY-P80405) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80405, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins (PubMed:10929008). Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity)

Subcellular Localization：Endoplasmic reticulum

Isoforms & Post-Translational Modification：P14314 has 2 isomers: P14314-1: 59425 Da (predicted); P14314-2: 59178 Da (predicted).

Subunit：Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH) (PubMed:10929008). Binds glycosylated PTPRC (By similarity)

RRID

AB_3102489

Database

Entrez Gene: 5589 Human

SwissProt: P14314 Human

OMIM: 174050 Human

Research Field

Tags & Cell Markers

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Glucosidase 2 subunit beta Antibody (YA409) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Glucosidase 2 subunit beta Antibody (YA409)

Glucosidase 2 subunit beta Antibody (YA409) Featured Recommendations:

Product Detail

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Background

Documentation

Glucosidase 2 subunit beta Antibody (YA409) Related Classifications

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