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PSAP Antibody (YA2683)

(Synonyms: PSAP; GLBA; SAP1; Proactivator polypeptide)
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Based on 1 Customer Validation

PSAP Antibody (YA2683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSAP.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • PSAP Antibody (YA2683) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PSAP Antibody (YA2683) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PSAP.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 78 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database

Entrez Gene: 5660 Human

SwissProt: P07602 Human

Immunogen

A synthesized peptide derived from human PSAP aa50-105/524.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105057

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PSAP Antibody (YA2683)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), PC-3 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), A431 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and 293 (lane 6, 15 μg) using PSAP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PSAP Antibody (YA2683)
    Western blot analysis of extracts from HepG2(lane 2(20ug) ，U-87MG(lane 3(20ug) and MCF-7(lane 4(20ug) using PSAP Antibody(HY-P82938) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PSAP is a Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate; Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases; Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12); Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling; Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases

  • Subcellular Localization

    Lysosome; Secreted

  • Isoforms & Post-Translational Modification

    P07602 has 3 isomers: P07602-1: 58113 Da (predicted); P07602-2: 58356 Da (predicted); P07602-3: 58484 Da (predicted).
    The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins;N-linked glycans show a high degree of microheterogeneity;The one residue extended Saposin-B-Val is only found in 5% of the chains

  • Subunit

    Saposin-B is a homodimer. Prosaposin exists as a roughly half-half mixture of monomers and disulfide-linked dimers (PubMed:10406958, PubMed:12510003, PubMed:21835174, PubMed:7730378). Monomeric prosaposin interacts (via C-terminus) with sortilin/SORT1, the interaction is required for targeting to lysosomes (PubMed:14657016, PubMed:22431521). Interacts with GRN; facilitates lysosomal delivery of progranulin from the extracellular space and the biosynthetic pathway (PubMed:26370502)

  • SwissProt ID

    P07602

  • Gene ID
    5660 [NCBI]
  • Synonyms

    PSAP; GLBA; SAP1; Proactivator polypeptide

  • Research Field

    Signal Transduction

PSAP Antibody (YA2683) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), PC-3 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), A431 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and 293 (lane 6, 15 μg) using PSAP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) ，U-87MG(lane 3(20ug) and MCF-7(lane 4(20ug) using PSAP Antibody(HY-P82938) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PSAP Antibody (YA2683) Product Image
    1/16
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • PSAP Antibody (YA2683) Product Image
  • Experimental Validation Results for PSAP Antibody (YA2683)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), PC-3 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), A431 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and 293 (lane 6, 15 μg) using PSAP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PSAP Antibody (YA2683)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2(lane 2(20ug) ，U-87MG(lane 3(20ug) and MCF-7(lane 4(20ug) using PSAP Antibody(HY-P82938) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • Experimental Validation Results for PSAP Antibody (YA2683)
  • PSAP Antibody (YA2683) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), PC-3 (lane 2, 15 μg), HepG2 (lane 3, 15 μg), A431 (lane 4, 15 μg), MCF-7 (lane 5, 15 μg), and 293 (lane 6, 15 μg) using PSAP Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:4000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PSAP Antibody (YA2683) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2(lane 2(20ug) ，U-87MG(lane 3(20ug) and MCF-7(lane 4(20ug) using PSAP Antibody(HY-P82938) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PSAP Antibody (YA2683) Product Image WB
  • PSAP Antibody (YA2683) Product Image WB