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YKL-40/CHI3L1 Antibody (YA6959)

(Synonyms: 39 kDa synovial protein antibody; ASRT7 antibody; Cartilage glycoprotein 39 antibody; CGP-39 antibody; CGP39 antibody; CH3L1_HUMAN antibody; CHI3L1 antibody; chitinase 3 like 1 (cartilage glycoprotein 39) antibody; chitinase 3 like 1 antibody; Chitinase 3 like protein 1 precursor antibody)
Customer Review

Based on 1 Customer Validation

YKL-40/CHI3L1 Antibody (YA6959) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to YKL-40/CHI3L1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200

Product Details

Description

YKL-40/CHI3L1 Antibody (YA6959) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to YKL-40/CHI3L1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Recombinant protein within extracellular domain of Human CHI3L1.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    YKL-40/CHI3L1 is a Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung

  • Subcellular Localization

    Secreted, extracellular space,Cytoplasm,Cytoplasm, perinuclear region,Endoplasmic reticulum

  • Expression


    Tissue_Specificity: Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts
    Induction: Up-regulated in colon under several inflammatory conditions. Down-regulated by hyperoxia in bronchial epithelial cells

  • Isoforms & Post-Translational Modification

    P36222: 383 amino acids, molecular weight 42625 Da.

  • Subunit

    Monomer

  • SwissProt ID

    P36222

  • Synonyms

    39 kDa synovial protein antibody; ASRT7 antibody; Cartilage glycoprotein 39 antibody; CGP-39 antibody; CGP39 antibody; CH3L1_HUMAN antibody; CHI3L1 antibody; chitinase 3 like 1 (cartilage glycoprotein 39) antibody; chitinase 3 like 1 antibody; Chitinase 3 like protein 1 precursor antibody

YKL-40/CHI3L1 Antibody (YA6959) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
    1/16
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • Experimental Validation Results for YKL-40/CHI3L1 Antibody (YA6959)
  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • YKL-40/CHI3L1 Antibody (YA6959) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using YKL-40/CHI3L1 Antibody (HY-P87276, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • YKL-40/CHI3L1 Antibody (YA6959) Product Image IHC-P
  • YKL-40/CHI3L1 Antibody (YA6959) Product Image IHC-P
  • YKL-40/CHI3L1 Antibody (YA6959) Product Image IHC-P