LAT Antibody (YA3161)
(Synonyms: LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38)LAT Antibody (YA3161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LAT.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
LAT Antibody (YA3161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LAT.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 36 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 28 kDa
A synthetic peptide of human LAT
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
LAT is required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules[1][2].
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Subcellular Localization
Cell membrane; Single-pass type III membrane protein
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Expression
Tissue_specificity:It is expressed in the thymus, T cells, NK cells, and mast cells, with lower expression levels in the spleen. It is present in T cells but not in B cells (at the protein level) . -
Isoforms & Post-Translational Modification
O43561 has 5 isomers: O43561-1: 27930 Da (predicted); O43561-2: 24985 Da (predicted); O43561-3: 28568 Da (predicted); O43561-4: 24829 Da (predicted); O43561-5: 27774 Da (predicted).
Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes;Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation;'Lys-63'-linked ubiquitinated by TRAF6 -
Subunit
When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity). Interacts with THEMIS2 (By similarity)
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SwissProt ID
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Synonyms
LAT; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38
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Research Field
Immunology
Documentation
[1]. Xie JJ, et al. TNFR-associated factor 6 regulates TCR signaling via interaction with and modification of LAT adapter. J Immunol. 2013 Apr 15;190(8):4027-36. [Content Brief]
[2]. Yang Y, et al. The Us3 Protein of Herpes Simplex Virus 1 Inhibits T Cell Signaling by Confining Linker for Activation of T Cells (LAT) Activation via TRAF6 Protein. J Biol Chem. 2015 Jun 19;290(25):15670-15678. [Content Brief]
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