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SUMO2/3 Antibody (YA1048)

(Synonyms: SUMO2; HSMT3; SMT3 homolog 2; SMT3A; Sentrin 2; Smt3B; SMT3H2; SUMO-2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Ubiquitin-like protein SMT3B)
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Based on 1 publication(s) in Google Scholar

SUMO2/3 Antibody (YA1048) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SUMO2/3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SUMO2/3 Antibody (YA1048)
  • SUMO2/3 Antibody (YA1048) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

SUMO2/3 Antibody (YA1048) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SUMO2/3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: Refer to figures Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Species Reactivity Database

Entrez Gene: 6613 Human

SwissProt: P61956 Human

Immunogen

Purified recombinant human SUMO-2/3 protein fragments expressed in E.coli.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3103419

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SUMO2/3 Antibody (YA1048)
    Western blot analysis of extracts from HeLa(lane 2, 20μg), HEK-293(lane 3, 20μg), HepG2(lane 4, 20μg) and Jurkat(lane 5, 20μg) using SUMO2/3 Antibody (HY-P81309). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SUMO2/3 is an Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus; Nucleus, PML body

  • Expression


    Tissue_specificity:Broad expression

  • Isoforms & Post-Translational Modification

    P61956 has 2 isomers: P61956-1: 10871 Da (predicted); P61956-2: 8111 Da (predicted).
    Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4;Cleavage of precursor form by SENP1 or SENP2 is necessary for function;Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer

  • Subunit

    Interacts with SAE2 and UBE2I. Interacts with ZNF451. Identified in a complex with ZNF451 and UBE2I/UBC9, where one ZNF451 interacts with one UBE2I/UBC9 and two SUMO2 chains, one bound to the UBE2I/UBC9 active site and the other to another region of the same UBE2I/UBC9 molecule.

  • SwissProt ID

    P61956

  • Gene ID
    6613 [NCBI]
  • Synonyms

    SUMO2; HSMT3; SMT3 homolog 2; SMT3A; Sentrin 2; Smt3B; SMT3H2; SUMO-2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Ubiquitin-like protein SMT3B

  • Research Field

    Cell Biology

SUMO2/3 Antibody (YA1048) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2, 20μg), HEK-293(lane 3, 20μg), HepG2(lane 4, 20μg) and Jurkat(lane 5, 20μg) using SUMO2/3 Antibody (HY-P81309). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • SUMO2/3 Antibody (YA1048) Product Image
    1/16
  • Experimental Validation Results for SUMO2/3 Antibody (YA1048)
  • SUMO2/3 Antibody (YA1048) Product Image
  • Experimental Validation Results for SUMO2/3 Antibody (YA1048)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa(lane 2, 20μg), HEK-293(lane 3, 20μg), HepG2(lane 4, 20μg) and Jurkat(lane 5, 20μg) using SUMO2/3 Antibody (HY-P81309). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SUMO2/3 Antibody (YA1048)
  • SUMO2/3 Antibody (YA1048) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa(lane 2, 20μg), HEK-293(lane 3, 20μg), HepG2(lane 4, 20μg) and Jurkat(lane 5, 20μg) using SUMO2/3 Antibody (HY-P81309). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

  • SUMO2/3 Antibody (YA1048) Product Image WB