SUMO2/3 Antibody (YA1048)

Cat. No.: HY-P81309: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

SUMO2/3 Antibody (YA1048) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SUMO2/3.

For research use only. We do not sell to patients.

Size	Stock	Quantity
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10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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SUMO2/3 Antibody (YA1048) Featured Recommendations:

1 Publications Citing Use of MCE SUMO2/3 Antibody (YA1048)

•Biofactors. 2026 Mar-Apr;52(2):e70086.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SUMO2/3 Antibody (YA1048) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to SUMO2/3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: Refer to figures

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P61956

Gene ID

6613 [NCBI]

Immunogen

Purified recombinant human SUMO-2/3 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HeLa(lane 2, 20μg), HEK-293(lane 3, 20μg), HepG2(lane 4, 20μg) and Jurkat(lane 5, 20μg) using SUMO2/3 Antibody (HY-P81309). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

Background

Function：Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451 (PubMed:26524494). This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (PubMed:18408734, PubMed:18538659, PubMed:21965678, PubMed:9556629). Plays a role in the regulation of sumoylation status of SETX (PubMed:24105744)

Subcellular Localization：Nucleus; Nucleus, PML body

Expression：
Tissue_specificity:Broad expression

Isoforms & Post-Translational Modification：P61956 has 2 isomers: P61956-1: 10871 Da (predicted); P61956-2: 8111 Da (predicted).
Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4;Cleavage of precursor form by SENP1 or SENP2 is necessary for function;Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer

Subunit：Interacts with SAE2 and UBE2I. Interacts with ZNF451. Identified in a complex with ZNF451 and UBE2I/UBC9, where one ZNF451 interacts with one UBE2I/UBC9 and two SUMO2 chains, one bound to the UBE2I/UBC9 active site and the other to another region of the same UBE2I/UBC9 molecule.

RRID

AB_3103419

Database

Entrez Gene: 6613 Human

SwissProt: P61956 Human

OMIM: 603042 Human

Research Field

Cell Biology

Synonyms

SUMO2; HSMT3; SMT3 homolog 2; SMT3A; Sentrin 2; Smt3B; SMT3H2; SUMO-2; SUMO-3; Sentrin-2; Ubiquitin-like protein SMT3A; Ubiquitin-like protein SMT3B

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

SUMO2/3 Antibody (YA1048) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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SUMO2/3 Antibody (YA1048)

SUMO2/3 Antibody (YA1048) Featured Recommendations:

1 Publications Citing Use of MCE SUMO2/3 Antibody (YA1048)

Product Detail

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Background

Documentation

SUMO2/3 Antibody (YA1048) Related Classifications

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