BID Antibody (YA4107)(PBS only)

Cat. No.: HY-P84410A: Data Sheet User Guide for Antibodies
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BID Antibody (YA4107) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BID.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

BID Antibody (YA4107) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to BID.

Host

Mouse

Molecular Weight

Predicted band size: 22 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P55957

Gene ID

637 [NCBI]

Immunogen

Purified recombinant full length protein of human BID full(aa 1-195).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity); Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622); Induces ICE-like proteases and apoptosis; Induces ICE-like proteases and apoptosis; Does not induce apoptosis; Induces ICE-like proteases and apoptosis

Subcellular Localization：Cytoplasm; Mitochondrion membrane; Mitochondrion outer membrane; Mitochondrion membrane; Mitochondrion membrane; Cytoplasm; Cytoplasm; Mitochondrion membrane

Expression：
Tissue_specificity:It is expressed in the spleen, pancreas, and placenta (protein level) ; it is expressed in the lungs, pancreas, and spleen (protein level) ; it is expressed in the lungs and pancreas (protein level) .

Isoforms & Post-Translational Modification：P55957 has 4 isomers: P55957-1: 21995 Da (predicted); P55957-2: 26836 Da (predicted); P55957-3: 14620 Da (predicted); P55957-4: 11263 Da (predicted).
TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622);Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation

Subunit：Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2 (By similarity). Interacts with PLEKHN1 (PubMed:29531808)

Synonyms

FP497; MGC15319; MGC42355; BID

Documentation

User Guide for Antibodies (1077 KB)