RNH1 (Ribonuclease Inhibitor) Antibody (YA8142)

Cat. No.: HY-P88458: Ficha de datos COA
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RNH1 (Ribonuclease Inhibitor) Antibody (YA8142) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNH1 (Ribonuclease Inhibitor).

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

RNH1 (Ribonuclease Inhibitor) Antibody (YA8142) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RNH1 (Ribonuclease Inhibitor).

Host

Mouse

Clonality

Monoclonal

Peso molecular

Predicted band size: 49.8 kDa

Species Reactivity

Human

SwissProt ID

P13489

Immunogen

Full length human recombinant protein of human RNH1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Pureza	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2, 20μg), HeLa(lane 3, 20μg), Jurkat(lane 4, 20μg) and A549(lane 5, 20μg) using RNH1 Antibody (HY-P88458). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hours at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10000) was used for 1 hour at room temperature.

Background

Function：Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG) (PubMed:12578357, PubMed:14515218, PubMed:3219362, PubMed:3243277, PubMed:3470787, PubMed:9050852). May play a role in redox homeostasis (PubMed:17292889). Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress (PubMed:23843625, PubMed:32510170). Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus (PubMed:23843625)

Subcellular Localization：Cytoplasm,Nucleus

Isoforms & Post-Translational Modification：P13489: 461 amino acids, molecular weight 49973 Da.

Subunit：Forms high-affinity heterodimers with RNASE1, ANG and RNASE2

Synonyms

RAI; RNH

Documentación

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Ficha de datos (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)