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Notch 2 Antibody (YA6134)

(Synonyms: NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2)
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Based on 1 Customer Validation

Notch 2 Antibody (YA6134) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Notch 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Notch 2 Antibody (YA6134)
  • Notch 2 Antibody (YA6134) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:100-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Notch 2 Antibody (YA6134) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Notch 2.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 120 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 265 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Notch 2 Antibody (YA6134)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) 、Rat liver (lane 4(20μg)) and Rat brain (lane 5(20μg)) using Notch2 Antibody (HY-P86442) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:It is expressed in the brain, heart, kidneys, lungs, skeletal muscle, and liver. It is widely expressed in embryos.

  • Subunit

    Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH2. Interacts with RELA/p65 (By similarity). Interacts with HIF1AN. Interacts (via ANK repeats) with TCIM, the interaction inhibits the nuclear translocation of NOTCH2 N2ICD (PubMed:25985737). Interacts with CUL1, RBX1, SKP1 and FBXW7 that are SCF(FBXW7) E3 ubiquitin-protein ligase complex components (PubMed:29149593). Interacts with MINAR1; this interaction increases MINAR1 stability and function (PubMed:29329397). Interacts with NOTCH2NL (NOTCH2NLA, NOTCH2NLB and/or NOTCH2NLC); leading to enhance Notch signaling pathway in a non-cell-autonomous manner (PubMed:29856954). Interacts with MDK; this interaction mediates a nuclear accumulation of NOTCH2 and therefore activation of NOTCH2 signaling leading to interaction between HES1 and STAT3 (PubMed:18469519). Interacts with MINAR2 (PubMed:32954300)

  • SwissProt ID

    Q04721

  • Gene ID
    4853 [NCBI]
  • Synonyms

    NOTCH2; Neurogenic locus notch homolog protein 2; Notch 2; hN2

Notch 2 Antibody (YA6134) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) 、Rat liver (lane 4(20μg)) and Rat brain (lane 5(20μg)) using Notch2 Antibody (HY-P86442) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Notch 2 Antibody (YA6134) Product Image
    1/16
  • Experimental Validation Results for Notch 2 Antibody (YA6134)
  • Notch 2 Antibody (YA6134) Product Image
  • Experimental Validation Results for Notch 2 Antibody (YA6134)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) 、Rat liver (lane 4(20μg)) and Rat brain (lane 5(20μg)) using Notch2 Antibody (HY-P86442) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Notch 2 Antibody (YA6134)
  • Notch 2 Antibody (YA6134) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) 、Rat liver (lane 4(20μg)) and Rat brain (lane 5(20μg)) using Notch2 Antibody (HY-P86442) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Notch 2 Antibody (YA6134) Product Image WB