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Galectin-3 Antibody (YA421)

(Synonyms: Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene,included; Galectin3; GALIG; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; Laminin binding protein; Laminin-binding protein; Lectin galactose binding soluble 3; Lectin galactoside binding soluble 3; Lectin L 29; Lectin L-29; Lectin, galactose binding, soluble 3; LGALS 3; LGALS2; LGALS3; LGALS3 protein; MAC 2 antigen; Mac-2; MAC2; Macrophage galactose-specific lectin; MGC105387; LEG3_HUMAN. )
Customer Review

Based on 1 Customer Validation

Galectin-3 Antibody (YA421) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Galectin-3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Galectin-3 Antibody (YA421) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200 1:50-1:100 1:200-1:1000

Product Details

Description

Galectin-3 Antibody (YA421) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Galectin-3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Galectin 3.AA range:1-49.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102488

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Galectin-3 Antibody (YA421)
    Western blot analysis of extracts from Hela using Galectin 3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
    Flow cytometric analysis of 1X106 HeLa cells labeling Galectin-3 Antibody (HY-P80404, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Galectin-3 is a β-galactoside-binding lectin that regulates cell adhesion, activation, migration, signaling, apoptosis, immune reactions, and neoplastic transformation[1][2]. Mechanistically, its conserved carbohydrate-recognition domain binds β-galactosides, while its intrinsically disordered N-terminal region enables multisite self-association and functional multivalency[1][2]. In inflammation, galectin-3 acts across acute inflammation, chronic inflammation, and tissue fibrogenesis, making it relevant to fibrosis-oriented experimental design[3]. In lung fibrosis models, galectin-3 deficiency or pharmacological inhibition reduced TGF-β1-driven epithelial-mesenchymal transition, myofibroblast activation, and collagen production[4]. More recent human lung fibroblast and IPF lung-slice data show that extracellular galectin-3 promotes integrin-mediated TGF-β1 activation and interacts with αv integrins and TGFβRII in a glycosylation-dependent manner[5]. Compared with related isoforms, galectin-3 is distinguished as the only vertebrate chimera-type galectin, containing one CRD linked to a long proline- and glycine-rich N-terminal domain[1]. For experimental applications, galectin-3 inhibitors that target the carbohydrate-recognition domain blocked integrin colocalization and reduced fibrotic mediators in IPF-derived lung tissue models[5]. In patients with idiopathic pulmonary fibrosis, inhaled TD139 showed target engagement and supported galectin-3 inhibition as a translational research approach[6].- Isoform distinction: galectin-3 differs structurally from prototype and tandem-repeat galectins through its chimera architecture[1]. - Research application: galectin-3 inhibition supports fibrosis studies involving TGF-β1 activation, integrins, and IPF models[4][5][6].

  • Subcellular Localization

    Cytoplasm; Nucleus; Secreted

  • Expression


    Tissue_specificity:This gene is mainly expressed in colonic epithelial cells, and is also abundant in activated macrophages and expressed in the fetal membranes.

  • Subunit

    Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM (PubMed:24945728). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059)

  • SwissProt ID

    P17931

  • Gene ID
    3958 [NCBI]
  • Synonyms

    Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene,included; Galectin3; GALIG; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; Laminin binding protein; Laminin-binding protein; Lectin galactose binding soluble 3; Lectin galactoside binding soluble 3; Lectin L 29; Lectin L-29; Lectin, galactose binding, soluble 3; LGALS 3; LGALS2; LGALS3; LGALS3 protein; MAC 2 antigen; Mac-2; MAC2; Macrophage galactose-specific lectin; MGC105387; LEG3_HUMAN.

  • Research Field

    Neuroscience

[1]. Dumic J, et al. Galectin-3: an open-ended story. Biochim Biophys Acta. 2006 Apr;1760(4):616-35. [Content Brief]

[2]. Lin YH, et al. The intrinsically disordered N-terminal domain of galectin-3 dynamically mediates multisite self-association of the protein through fuzzy interactions. J Biol Chem. 2017 Oct 27;292(43):17845-17856. [Content Brief]

[3]. Henderson NC, et al. The regulation of inflammation by galectin-3. Immunol Rev. 2009 Jul;230(1):160-71. [Content Brief]

[4]. Mackinnon AC, et al. Regulation of transforming growth factor-β1-driven lung fibrosis by galectin-3. Am J Respir Crit Care Med. 2012 Mar 1;185(5):537-46. [Content Brief]

[5]. Calver JF, et al. Defining the mechanism of galectin-3-mediated TGF-β1 activation and its role in lung fibrosis. J Biol Chem. 2024 Jun;300(6):107300. [Content Brief]

[6]. Hirani N, et al. Target inhibition of galectin-3 by inhaled TD139 in patients with idiopathic pulmonary fibrosis. Eur Respir J. 2021 May 27;57(5):2002559. [Content Brief]

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Galectin-3 Antibody (YA421) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela using Galectin 3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Galectin-3 Antibody (HY-P80404, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Galectin-3 Antibody (YA421) Product Image
    1/16
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Galectin-3 Antibody (YA421) Product Image
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela using Galectin 3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Galectin-3 Antibody (HY-P80404, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Experimental Validation Results for Galectin-3 Antibody (YA421)
  • Galectin-3 Antibody (YA421) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela using Galectin 3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Galectin-3 Antibody (YA421) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling Galectin-3 Antibody (HY-P80404, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Galectin-3 Antibody (YA421) Product Image WB
  • Galectin-3 Antibody (YA421) Product Image FC