TIAM2 Antibody (YA2200)(PBS only)

Cat. No.: HY-P82455A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

TIAM2 Antibody (YA2200) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIAM2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TIAM2 Antibody (YA2200) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIAM2.

Host

Rabbit

Molecular Weight

Predicted band size: 190 kDa;

Observed band size: 190 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8IVF5

Gene ID

26230 [NCBI]

Immunogen

Recombinant protein of human TIAM2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA

Subcellular Localization：Cytoplasm; Cell projection, lamellipodium; Cell projection, filopodium; Cell projection, growth cone; Cell projection, neuron projection; Perikaryon

Expression：
Tissue_specificity:Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis

Isoforms & Post-Translational Modification：Q8IVF5 has 5 isomers: Q8IVF5-1: 190103 Da (predicted); Q8IVF5-2: 193398 Da (predicted); Q8IVF5-3: 70774 Da (predicted); Q8IVF5-4: 114079 Da (predicted); Q8IVF5-5: 192555 Da (predicted).
Phosphorylated on serine and threonine residues. Phosphorylated on Thr-1648 by Rho-kinase. Its phosphorylation by Rho-kinase inhibits its guanine nucleotide exchange activity, its interaction with MAP1A, MAP1B, PARP1 and YWHAE and reduces its ability to promote neurite growth (By similarity)

Subunit：Interacts with MAP1A, MAP1B, PARP1 and YWHAE. Interacts with CD44, PARD3 and MAPK8IP2 (By similarity)

Research Field

Signal Transduction

Synonyms

STEF; TIAM-2

Documentation

User Guide for Antibodies (1077 KB)