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C-erbB-3/HER3 Antibody (YA457)

(Synonyms: ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3)
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Based on 1 Customer Validation

C-erbB-3/HER3 Antibody (YA457) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-3/HER3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
WB
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • C-erbB-3/HER3 Antibody (YA457) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:100-1:200 1:20-1:50

Product Details

Description

C-erbB-3/HER3 Antibody (YA457) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to C-erbB-3/HER3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 185 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 148 kDa
Species Reactivity Database

Entrez Gene: 2065 Human

SwissProt: P21860 Human

Immunogen

Synthetic peptide corresponding to Human ErbB3 aa1094-1111.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using ErbB3 / HER3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 1), Mouse brain (lane 2), and MCF-7 (lane 3) using C-erbB-3/HER3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:500) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), Mouse skin (lane 4, 20ug) and K562 (lane 5, 20ug) using C-erbB-3/HER3 Antibody (YA457) (HY-P80659). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908)

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:Epithelial tissue and brain

  • Isoforms & Post-Translational Modification

    P21860 has 5 isomers: P21860-1: 148098 Da (predicted); P21860-2: 20136 Da (predicted); P21860-3: 36490 Da (predicted); P21860-4: 141752 Da (predicted); P21860-5: 77426 Da (predicted).
    Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778)

  • Subunit

    Monomer and homodimer. Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 (PubMed:15358134). Interacts with GRB7 (PubMed:9516479). Interacts with MUC1 (PubMed:12939402). Interacts with MYOC (By similarity). Interacts with isoform 2 of PA2G4 (PubMed:11325528, PubMed:16832058). Found in a ternary complex with NRG1 and ITGAV:ITGB3 or ITGA6:ITGB4 (PubMed:20682778)

  • SwissProt ID

    P21860

  • Gene ID
    2065 [NCBI]
  • Synonyms

    ERBB3; HER3; Receptor tyrosine-protein kinase erbB-3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3

  • Research Field

    Cell Biology

C-erbB-3/HER3 Antibody (YA457) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using ErbB3 / HER3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 1), Mouse brain (lane 2), and MCF-7 (lane 3) using C-erbB-3/HER3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:500) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), Mouse skin (lane 4, 20ug) and K562 (lane 5, 20ug) using C-erbB-3/HER3 Antibody (YA457) (HY-P80659). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • C-erbB-3/HER3 Antibody (YA457) Product Image
    1/16
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • C-erbB-3/HER3 Antibody (YA457) Product Image
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using ErbB3 / HER3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 1), Mouse brain (lane 2), and MCF-7 (lane 3) using C-erbB-3/HER3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:500) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), Mouse skin (lane 4, 20ug) and K562 (lane 5, 20ug) using C-erbB-3/HER3 Antibody (YA457) (HY-P80659). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • Experimental Validation Results for C-erbB-3/HER3 Antibody (YA457)
  • C-erbB-3/HER3 Antibody (YA457) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using ErbB3 / HER3 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • C-erbB-3/HER3 Antibody (YA457) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 1), Mouse brain (lane 2), and MCF-7 (lane 3) using C-erbB-3/HER3 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using a wet transfer system for 90 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:500) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • C-erbB-3/HER3 Antibody (YA457) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2, 20ug), SK-Br-3 (lane 3, 20ug), Mouse skin (lane 4, 20ug) and K562 (lane 5, 20ug) using C-erbB-3/HER3 Antibody (YA457) (HY-P80659). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • C-erbB-3/HER3 Antibody (YA457) Product Image WB
  • C-erbB-3/HER3 Antibody (YA457) Product Image WB
  • C-erbB-3/HER3 Antibody (YA457) Product Image WB