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p27 KIP 1 Antibody (YA6248)

(Synonyms: CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1)
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Based on 1 Customer Validation

p27 KIP 1 Antibody (YA6248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p27 KIP 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for p27 KIP 1 Antibody (YA6248)
  • p27 KIP 1 Antibody (YA6248) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:1000-1:5000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

p27 KIP 1 Antibody (YA6248) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p27 KIP 1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 27 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for p27 KIP 1 Antibody (YA6248)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Neuro-2a (lane 3(20μg)) using p27 KIP 1 Antibody (HY-P86556) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    p27 KIP 1 is important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry[1].

  • Subcellular Localization

    Nucleus; Cytoplasm; Endosome

  • Expression


    Tissue_specificity:It is expressed in the kidney (protein level) (PubMed: 15509543) . It is expressed in all tissues tested (PubMed: 8033212) . The highest content is found in skeletal muscle, and the lowest content is found in the liver and kidney (PubMed: 8033212) .

    Induction:Maximal levels in quiescence cells and early G (1) . Levels decrease after mitogen stimulation as cells progress toward S-phase

  • Subunit

    Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.

  • SwissProt ID

    P46527

  • Gene ID
    1027 [NCBI]
  • Synonyms

    CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

References

[1]. McGrath DA, et al. Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins. EMBO J. 2017 Aug 1;36(15):2251-2262. [Content Brief]

p27 KIP 1 Antibody (YA6248) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Neuro-2a (lane 3(20μg)) using p27 KIP 1 Antibody (HY-P86556) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • p27 KIP 1 Antibody (YA6248) Product Image
    1/16
  • Experimental Validation Results for p27 KIP 1 Antibody (YA6248)
  • p27 KIP 1 Antibody (YA6248) Product Image
  • Experimental Validation Results for p27 KIP 1 Antibody (YA6248)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Neuro-2a (lane 3(20μg)) using p27 KIP 1 Antibody (HY-P86556) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for p27 KIP 1 Antibody (YA6248)
  • p27 KIP 1 Antibody (YA6248) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、MCF7 (lane 2(20μg)) and Neuro-2a (lane 3(20μg)) using p27 KIP 1 Antibody (HY-P86556) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • p27 KIP 1 Antibody (YA6248) Product Image WB