PRPF8 Antibody (YA1759)

(Synonyms: HPRP8; p220; PRP8; PRPC8; Prpf8; RP13; SNRNP220)
Customer Review

Based on 1 Customer Validation

PRPF8 Antibody (YA1759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

PRPF8 Antibody (YA1759) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PRPF8.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 274 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 274 kDa
Species Reactivity Database

Entrez Gene: 10594 Human

SwissProt: Q6P2Q9 Human

Immunogen

A synthetic peptide of human PRPF8

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Western blot analysis of extracts from HEK293T (lane 2(20μg), SiHa (lane 3(20μg), A549 (lane 4(20μg), K562 (lane 5(20μg) using PRPF8 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PRPF8 Antibody (YA1759)
    Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using PRPF8 Antibody (HY-P82014, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    PRPF8 plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Nucleus; Nucleus speckle

  • Expression


    Tissue_specificity:Widely expressed

  • Subunit

    Part of the U5 snRNP complex (PubMed:2527369, PubMed:2532307). Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (PubMed:16723661, PubMed:2479028, PubMed:26912367). Component of the U5.U4atac/U6atac snRNP complexes in U12-dependent spliceosomes (PubMed:11971955). Within the minor spliceosome, which acts on U12-type introns, interacts with PPIL2 and RBM48 (PubMed:33509932). Core component of U2-type precatalytic, catalytic and postcatalytic spliceosomal complexes (PubMed:10411133, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:29301961, PubMed:29360106, PubMed:29361316, PubMed:30315277, PubMed:30705154, PubMed:30728453). Found in a mRNA splicing-dependent exon junction complex (EJC) with SRRM1. Interacts with U5 snRNP proteins SNRP116 and SNRNP40. Interacts with EFTUD2. Interacts (via the MPN (JAB/Mov34) domain) with PRPF3 ('Lys-63'-linked polyubiquitinated); may stabilize the U4/U6-U5 tri-snRNP complex. Interacts (via RNase H homology domain) with AAR2 (PubMed:26527271). Interacts with RPAP3 and URI1 in a ZNHIT2-dependent manner (PubMed:28561026). Interacts with C9orf78 (PubMed:35167828, PubMed:35241646). Interacts with SNRNP200; the interaction is direct (PubMed:35188580, PubMed:35241646). Interacts with TSSC4; the interaction is direct (PubMed:35188580)

  • SwissProt ID

    Q6P2Q9

  • Gene ID
  • Synonyms

    HPRP8; p220; PRP8; PRPC8; Prpf8; RP13; SNRNP220

  • Research Field

    Epigenetics and Nuclear Signaling

PRPF8 Antibody (YA1759) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00