BCLAF1 Antibody (YA7155)

Cat. No.: HY-P87468: Data Sheet COA
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User Guide for Antibodies
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BCLAF1 Antibody (YA7155) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCLAF1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

BCLAF1 Antibody (YA7155) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCLAF1.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 106kDa;

Observed band size: 150kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9NYF8

Immunogen

A synthesized peptide derived from human BTF: 60-120/920.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-1:200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Solution

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), MCF-7 (lane 3), HeLa (lane 4), A549 (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using BCLAF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

Function：Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA

Subcellular Localization：Cytoplasm,Nucleus,Nucleus speckle,Nucleus, nucleoplasm

Expression：
Tissue_Specificity: Ubiquitous

Isoforms & Post-Translational Modification：Q9NYF8 has four isomers: Q9NYF8-1: 106122 Da (predicted); Q9NYF8-2: 105948 Da (predicted); Q9NYF8-3: 100232 Da (predicted); Q9NYF8-4: 85937 Da (predicted).
Citrullinated by PADI4

Subunit：Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA

Synonyms

BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

Documentation

Select Batch:

Data Sheet (257 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)