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BCLAF1 Antibody (YA7155)

(Synonyms: BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1)
Revisión del cliente

Based on 1 Customer Validation

BCLAF1 Antibody (YA7155) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCLAF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BCLAF1 Antibody (YA7155)
  • BCLAF1 Antibody (YA7155) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
Dilution Ratio 1:500-2000 1:50-200 1:50-200 1:50-200 1:50-1:200

Product Details

Descripciòn

BCLAF1 Antibody (YA7155) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BCLAF1.

  • Host Rabbit
  • Clonality Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 150kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 106kDa
Immunogen

A synthesized peptide derived from human BTF: 60-120/920.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Envío

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BCLAF1 Antibody (YA7155)
    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), MCF-7 (lane 3), HeLa (lane 4), A549 (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using BCLAF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    BCLAF1 is a Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA

  • Subcellular Localization

    Cytoplasm,Nucleus,Nucleus speckle,Nucleus, nucleoplasm

  • Expression


    Tissue_Specificity: Ubiquitous

  • Isoforms & Post-Translational Modification

    Q9NYF8 has four isomers: Q9NYF8-1: 106122 Da (predicted); Q9NYF8-2: 105948 Da (predicted); Q9NYF8-3: 100232 Da (predicted); Q9NYF8-4: 85937 Da (predicted).
    Citrullinated by PADI4

  • Subunit

    Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA

  • SwissProt ID

    Q9NYF8

  • Synonyms

    BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

BCLAF1 Antibody (YA7155) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), MCF-7 (lane 3), HeLa (lane 4), A549 (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using BCLAF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BCLAF1 Antibody (YA7155) Product Image
    1/16
  • Experimental Validation Results for BCLAF1 Antibody (YA7155)
  • BCLAF1 Antibody (YA7155) Product Image
  • Experimental Validation Results for BCLAF1 Antibody (YA7155)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), MCF-7 (lane 3), HeLa (lane 4), A549 (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using BCLAF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for BCLAF1 Antibody (YA7155)
  • BCLAF1 Antibody (YA7155) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from 293T (lane 2), MCF-7 (lane 3), HeLa (lane 4), A549 (lane 5), HT-1080 (lane 6), and Caco-2 (lane 7) using BCLAF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • BCLAF1 Antibody (YA7155) Product Image WB