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NOX2 Antibody

(Synonyms: AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytochrome b 245, beta polypeptide; Cytochrome b(558) beta chain; Cytochrome b(558) subunit beta; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP91PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; P91 PHOX; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit.)
Customer Review

Based on 1 Customer Validation

NOX2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NOX2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NOX2 Antibody
  • NOX2 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1:100-500

Product Details

Description

NOX2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NOX2.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Chicken,Dog,Pig,Cow,Horse,Rabbit
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 65 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 65 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human NOX2: 501-570/570

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103095

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NOX2 Antibody
    Western blot analysis of extracts from RAW264.7 (lane 1) and M-Liver (lane 2) using NOX2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    NOX2 (NADPH oxidase 2) is a catalytic member of the NADPH oxidase family that is specialized for reactive oxygen species (ROS) generation and serves as a central component of phagocyte antimicrobial defense and redox signaling pathways[1][2]. NOX2 activation requires assembly of membrane and cytosolic regulatory subunits, enabling superoxide production at the plasma membrane or phagosomal membrane during immune responses[2]. Mechanistically, NOX2-derived ROS contribute not only to pathogen killing but also to immune regulation, antigen presentation, and maintenance of self-tolerance, linking oxidative signaling to both innate and adaptive immunity[3][4]. Dysregulated NOX2 activity has been implicated in multiple inflammatory and oxidative stress-associated disease models, while genetic deficiency of NOX2 causes chronic granulomatous disease characterized by impaired microbial killing and recurrent infections[3][5]. In disease models, NOX2-derived ROS have been associated with inflammation, fibrosis, and cardiovascular pathology, highlighting the broad biological relevance of this isoform[5][6]. Compared with related NOX isoforms, NOX2 is predominantly expressed in phagocytic cells, whereas NOX1, NOX3, NOX4, and NOX5 are primarily expressed in non-phagocytic tissues, indicating distinct physiological functions and cellular contexts[7]. For experimental applications, NOX inhibitors and NOX2-targeted inhibitory peptides such as NOX2ds-tat have been used to investigate NOX2-dependent signaling; however, limited isoform selectivity remains an important consideration when interpreting pharmacological studies[8].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Protein levels were detected in neutrophils.

  • Subunit

    Component of the phagocyte NADPH oxidase core complex/cytochrome b558 complex, composed of CYBB (heavy chain (beta)) and CYBA (light chain (alpha)) (PubMed:3600769, PubMed:36241643, PubMed:36413210). Component of the phagocyte NADPH oxidase complex composed of an obligatory core heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic regulatory subunits NCF1/p47-phox, NCF2/p67-phox, NCF4/p40-phox and the small GTPase RAC1 or RAC2 (PubMed:15338276, PubMed:19028840, PubMed:38355798). Interacts with NCF1 (phosphorylated form) (PubMed:19028840, PubMed:9224653). Interacts with NCF2; the interaction is enhanced in the presence of GBP7 (PubMed:19028840, PubMed:38355798). Interacts with RAC2 (PubMed:19028840). Interacts with RAC1 (PubMed:38355798). Interacts with calprotectin (S100A8/9) (PubMed:22808130). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity). Interacts with CYBC1; CYBC1 may act as a chaperone stabilizing Cytochrome b-245 heterodimer (PubMed:28351984). The CYBA-CYBB complex interacts with GBP7 (By similarity)

  • SwissProt ID

    P04839

  • Gene ID
    1536 [NCBI]
  • Synonyms

    AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytochrome b 245, beta polypeptide; Cytochrome b(558) beta chain; Cytochrome b(558) subunit beta; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP91PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; P91 PHOX; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit.

[1]. Cipriano A, et al. NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors. J Med Chem. 2023 Sep 14;66(17):11632-11655. [Content Brief]

[2]. Paclet MH, et al. Regulation of Neutrophil NADPH Oxidase, NOX2: A Crucial Effector in Neutrophil Phenotype and Function. Front Cell Dev Biol. 2022 Jul 14;10:945749. [Content Brief]

[3]. Altenhöfer S, et al. Evolution of NADPH Oxidase Inhibitors: Selectivity and Mechanisms for Target Engagement. Antioxid Redox Signal. 2015 Aug 10;23(5):406-27. [Content Brief]

NOX2 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 1) and M-Liver (lane 2) using NOX2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • NOX2 Antibody Product Image
    1/16
  • Experimental Validation Results for NOX2 Antibody
  • NOX2 Antibody Product Image
  • Experimental Validation Results for NOX2 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7 (lane 1) and M-Liver (lane 2) using NOX2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for NOX2 Antibody
  • NOX2 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7 (lane 1) and M-Liver (lane 2) using NOX2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • NOX2 Antibody Product Image WB