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NADPH Oxidase 4 Antibody

(Synonyms: NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX)
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Based on 1 publication(s) in Google Scholar

NADPH Oxidase 4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NADPH Oxidase 4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC ICC
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • NADPH Oxidase 4 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:20

Product Details

Description

NADPH Oxidase 4 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to NADPH Oxidase 4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67 kDa
Species Reactivity Database

Entrez Gene: 50507 Human

SwissProt: Q9NPH5 Human

Immunogen

Synthetic peptide corresponding to Human NOX4 aa510-578.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102700

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Western blot analysis of extracts from Hela using NADPH Oxidase 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    NADPH Oxidase 4 is a NADPH oxidase that catalyzes predominantly the reduction of oxygen to H2O2. Can also catalyze to a smaller extent, the reduction of oxygen to superoxide. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Promotes ferroptosis, reactive oxygen species production and reduced glutathione (GSH) levels by activating NLRP3 inflammasome activation and cytokine release; NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Involved in redox signaling in vascular cells. Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage; Lacks superoxide-generating NADPH oxidase activity[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16].

  • Subcellular Localization

    Cytoplasm; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein; Cell junction, focal adhesion; Nucleus; Nucleus; Nucleus, nucleolus; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:This gene is expressed (protein level) in distal renal tubular cells and endothelial cells of the renal cortex. Expression is widespread. It is highly expressed in the kidneys, and expressed at lower levels in the heart, adipocytes, liver cancer cells, endothelial cells, skeletal muscle, brain, various brain tumor cell lines, and airway epithelial cells.

    Induction:By 7-ketocholesterol (at protein level)

  • Isoforms & Post-Translational Modification

    Q9NPH5 has 9 isomers: Q9NPH5-1: 66932 Da (predicted); Q9NPH5-2: 58410 Da (predicted); Q9NPH5-3: 27625 Da (predicted); Q9NPH5-4: 31812 Da (predicted); Q9NPH5-5: 25761 Da (predicted); Q9NPH5-6: 62745 Da (predicted); Q9NPH5-7: 6516 Da (predicted); Q9NPH5-8: 64102 Da (predicted); Q9NPH5-9: 59915 Da (predicted).
    Deubiquitinated by USP19;N-glycosylated and glycosylation is required for its proper function;N-glycosylated

  • Subunit

    Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4. Interacts with PPP1R15A (PubMed:26742780). Interacts with LRRC8A; this interaction prevents the ubiquitin-mediated degradation of LRRC8A (PubMed:39909992)

  • SwissProt ID

    Q9NPH5

  • Gene ID
    50507 [NCBI]
  • Synonyms

    NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX

  • Research Field

    Cardiovascular

NADPH Oxidase 4 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela using NADPH Oxidase 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody Product Image
    1/16
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • NADPH Oxidase 4 Antibody Product Image
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela using NADPH Oxidase 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • Experimental Validation Results for NADPH Oxidase 4 Antibody
  • NADPH Oxidase 4 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela using NADPH Oxidase 4 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • NADPH Oxidase 4 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Lung squamous cell carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NADPH Oxidase 4 Antibody Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue using NADPH Oxidase 4 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • NADPH Oxidase 4 Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • NADPH Oxidase 4 Antibody Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using NADPH Oxidase 4 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80762, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • NADPH Oxidase 4 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling NADPH Oxidase 4 with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with NADPH Oxidase 4 Antibody (HY-P80762) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • NADPH Oxidase 4 Antibody Product Image WB
  • NADPH Oxidase 4 Antibody Product Image IHC-P
  • NADPH Oxidase 4 Antibody Product Image IHC-P
  • NADPH Oxidase 4 Antibody Product Image mIHC
  • NADPH Oxidase 4 Antibody Product Image mIHC
  • NADPH Oxidase 4 Antibody Product Image ICC
  • NADPH Oxidase 4 Antibody Product Image ICC