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  5. VAV1 Antibody (YA2161)

VAV1 Antibody (YA2161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAV1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

  • Referencias

Descripciòn

VAV1 Antibody (YA2161) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VAV1.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Peso molecular
Predicted band size: 98 kDa;
Observed band size: 98 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human Vav1 aa750-800/845.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:50
FC
FC: Flow Cytometry
1:50-1:100
Sensitivity Endogenous Pureza Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), Ramos (lane 2, 20 μg), HL-60 (lane 3, 20 μg) using VAV1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation
Expression：
Tissue_specificity:It is widely expressed in hematopoietic cells, but not in other cell types.
Isoforms & Post-Translational Modification：P15498 has 2 isomers: P15498-1: 98314 Da (predicted); P15498-2: 94493 Da (predicted).
Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS) (By similarity). Phosphorylated by FYN
Subunit：Interacts with SHB (PubMed:12084069). Interacts with SH2B2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK (PubMed:12393632, PubMed:12400014, PubMed:15558030). Interacts with SIAH2; without leading to its degradation (PubMed:10207103). Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion (PubMed:9697839). Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity (PubMed:9399639). May interact with CCPG1. Interacts with CLNK. Interacts with THEMIS2 (By similarity). Interacts with NEK3 and this interaction is prolactin-dependent (PubMed:15618286). Interacts with ITK (PubMed:15661896). Interacts with PTK2B/PYK2 (By similarity). Interacts with HCK. Interacts with PTK2B/PYK2 (PubMed:19207108). Interacts (via SH2 domain) with SYK (PubMed:8986718). Interacts with ANKRD54. Interacts with CD6 (By similarity). Interacts with isoform 2 of CRACR2A (PubMed:27016526). Interacts with LCP2; this interaction plays a role in TCR-mediated cytokine production (PubMed:8673706)
RRID
Database

Entrez Gene: 7409 Human

SwissProt: P15498 Human

OMIM: 164875 Human

Research Field

Signal Transduction
Synonyms
p95; p95vav; Protein vav 1; VAV; vav-T
Documentación
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
Referencias

VAV1 Antibody (YA2161) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
VAV1 Antibody (YA2161)
Cat. No.:
HY-P82416
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