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Cyclin H Antibody (YA5224)

(Synonyms: 6330408H09Rik; AI661354; AV102684; AW538719; CAK; CAK complex subunit; ccnh; CCNH_HUMAN; CDK activating kinase; CDK activating kinase complex subunit; Cyclin dependent kinase activating kinase; cyclin dependent kinase activating kinase complex subunit; Cyclin H; CyclinH; MO15-associated protein; p34; p36; p37.)
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Based on 1 Customer Validation

Cyclin H Antibody (YA5224) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Cyclin H.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cyclin H Antibody (YA5224)
  • Cyclin H Antibody (YA5224) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Cyclin H Antibody (YA5224) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Cyclin H.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 38 kDa; Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Purified recombinant human Cyclin H protein fragments expressed in E.coli.

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3719026

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cyclin H Antibody (YA5224)
    Western blot analysis of extracts from K562(lane 2(20μg), Jurkat(lane 3(20μg), Hela (lane 4(20μg) and MCF-7(lane 5(20μg) using Cyclin H Antibody (HY-P85532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Cyclin H regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle

  • Subcellular Localization

    Nucleus

  • Subunit

    Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor

  • SwissProt ID

    P51946

  • Gene ID
    902 [NCBI]
  • Synonyms

    6330408H09Rik; AI661354; AV102684; AW538719; CAK; CAK complex subunit; ccnh; CCNH_HUMAN; CDK activating kinase; CDK activating kinase complex subunit; Cyclin dependent kinase activating kinase; cyclin dependent kinase activating kinase complex subunit; Cyclin H; CyclinH; MO15-associated protein; p34; p36; p37.

Cyclin H Antibody (YA5224) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), Jurkat(lane 3(20μg), Hela (lane 4(20μg) and MCF-7(lane 5(20μg) using Cyclin H Antibody (HY-P85532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cyclin H Antibody (YA5224) Product Image
    1/16
  • Experimental Validation Results for Cyclin H Antibody (YA5224)
  • Cyclin H Antibody (YA5224) Product Image
  • Experimental Validation Results for Cyclin H Antibody (YA5224)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg), Jurkat(lane 3(20μg), Hela (lane 4(20μg) and MCF-7(lane 5(20μg) using Cyclin H Antibody (HY-P85532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cyclin H Antibody (YA5224)
  • Cyclin H Antibody (YA5224) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg), Jurkat(lane 3(20μg), Hela (lane 4(20μg) and MCF-7(lane 5(20μg) using Cyclin H Antibody (HY-P85532). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Cyclin H Antibody (YA5224) Product Image WB