FGF-2 Antibody (YA442)

Cat. No.: HY-P80671: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

FGF-2 Antibody (YA442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF-2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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FGF-2 Antibody (YA442) Featured Recommendations:

1 Publications Citing Use of MCE FGF-2 Antibody (YA442)

•Biochem Pharmacol. 2026 May:247:117783.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

FGF-2 Antibody (YA442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF-2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 31 kDa;

Observed band size: 18/22/24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P09038

Gene ID

2247 [NCBI]

Immunogen

Synthetic peptide within Human FGF2 aa 121-170 / 288.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	1:50
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB FC

Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879)

Subcellular Localization：Secreted; Nucleus

Expression：
Tissue_specificity:It is expressed in granulosa cells and cumulus cells. It is expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

Isoforms & Post-Translational Modification：P09038 has 4 isomers: P09038-4: 30770 Da (predicted); P09038-1: 22623 Da (predicted); P09038-2: 17254 Da (predicted); P09038-3: 21203 Da (predicted).
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion;Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing

Subunit：Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP3 (PubMed:18669637). Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling (PubMed:28302677). Interacts with SNORC (via the extracellular domain) (By similarity). Interacts with glypican GPC3 (By similarity)

RRID

AB_3102657

Database

Entrez Gene: 2247 Human ; 14173 Mouse ;

SwissProt: P09038 Human ; P15655 Mouse ;

OMIM: 134920 Human

Research Field

Cardiovascular

Synonyms

FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

FGF-2 Antibody (YA442) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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FGF-2 Antibody (YA442)

FGF-2 Antibody (YA442) Featured Recommendations:

1 Publications Citing Use of MCE FGF-2 Antibody (YA442)

Product Detail

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Background

Documentation

FGF-2 Antibody (YA442) Related Classifications

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