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FGF-2 Antibody (YA442)

(Synonyms: FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2)
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Based on 1 publication(s) in Google Scholar

FGF-2 Antibody (YA442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF-2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • FGF-2 Antibody (YA442) Product Image
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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50 1:200-1:1000

Product Details

Description

FGF-2 Antibody (YA442) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGF-2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 18/22/24 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human FGF2 aa 121-170 / 288.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102657

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FGF-2 Antibody (YA442)
    Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGF-2 Antibody (YA442)
    Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879)

  • Subcellular Localization

    Secreted; Nucleus

  • Expression


    Tissue_specificity:It is expressed in granulosa cells and cumulus cells. It is expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.

  • Isoforms & Post-Translational Modification

    P09038 has 4 isomers: P09038-4: 30770 Da (predicted); P09038-1: 22623 Da (predicted); P09038-2: 17254 Da (predicted); P09038-3: 21203 Da (predicted).
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion;Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing

  • Subunit

    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP3 (PubMed:18669637). Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling (PubMed:28302677). Interacts with SNORC (via the extracellular domain) (By similarity). Interacts with glypican GPC3 (By similarity)

  • SwissProt ID

    P09038

  • Gene ID
    2247 [NCBI]
  • Synonyms

    FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

  • Research Field

    Cardiovascular

FGF-2 Antibody (YA442) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • FGF-2 Antibody (YA442) Product Image
    1/16
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • FGF-2 Antibody (YA442) Product Image
  • Experimental Validation Results for FGF-2 Antibody (YA442)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for FGF-2 Antibody (YA442)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • Experimental Validation Results for FGF-2 Antibody (YA442)
  • FGF-2 Antibody (YA442) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , K562 (lane 3(20μg) , and HEK293T (lane 4(20μg)using FGF2 (HY-P80671) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80671, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • FGF-2 Antibody (YA442) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Jurkat cells labeling FGF2 Antibody(HY-P80671, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • FGF-2 Antibody (YA442) Product Image WB
  • FGF-2 Antibody (YA442) Product Image FC