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ACSS1 Antibody (YA4525)

(Synonyms: ACAS2L; ACECS1; AceCS2L)
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Based on 1 Customer Validation

ACSS1 Antibody (YA4525) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSS1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACSS1 Antibody (YA4525)
  • ACSS1 Antibody (YA4525) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:10000

Product Details

Description

ACSS1 Antibody (YA4525) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACSS1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa
Immunogen

Purified recombinant fragment of human ACSS1 (AA: 548-689) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACSS1 Antibody (YA4525)
    Western blot analysis of extracts from Jurkat(lane 2(20ug), HL-60(lane 3(20ug) and HT-29(lane 4(20ug) , Raji(lane 5(20ug) using ACSS1 Antibody(HY-P84828) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ACSS1 catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity. Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions. Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient[1].

  • Subcellular Localization

    Mitochondrion matrix

  • Isoforms & Post-Translational Modification

    Q9NUB1 has 4 isomers: Q9NUB1-1: 74857 Da (predicted); Q9NUB1-2: 74627 Da (predicted); Q9NUB1-3: 62722 Da (predicted); Q9NUB1-4: 62688 Da (predicted).
    Reversibly acetylated on Lys-642 (PubMed:16788062). The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3

  • Subunit

    Interacts with SIRT3

  • SwissProt ID

    Q9NUB1

  • Gene ID
    84532 [NCBI]
  • Synonyms

    ACAS2L; ACECS1; AceCS2L

ACSS1 Antibody (YA4525) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug), HL-60(lane 3(20ug) and HT-29(lane 4(20ug) , Raji(lane 5(20ug) using ACSS1 Antibody(HY-P84828) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACSS1 Antibody (YA4525) Product Image
    1/16
  • Experimental Validation Results for ACSS1 Antibody (YA4525)
  • ACSS1 Antibody (YA4525) Product Image
  • Experimental Validation Results for ACSS1 Antibody (YA4525)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug), HL-60(lane 3(20ug) and HT-29(lane 4(20ug) , Raji(lane 5(20ug) using ACSS1 Antibody(HY-P84828) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ACSS1 Antibody (YA4525)
  • ACSS1 Antibody (YA4525) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug), HL-60(lane 3(20ug) and HT-29(lane 4(20ug) , Raji(lane 5(20ug) using ACSS1 Antibody(HY-P84828) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ACSS1 Antibody (YA4525) Product Image WB