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IRE1 Antibody (YA3557)

(Synonyms: IRE1; IRE1P; IRE1a; hIRE1p; FLJ30999; MGC163277; MGC163279; ERN1)
Customer Review

Based on 1 Customer Validation

IRE1 Antibody (YA3557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IRE1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • IRE1 Antibody (YA3557) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:10000

Product Details

Description

IRE1 Antibody (YA3557) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IRE1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Immunogen

Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718783

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Western blot analysis of extracts from HEK293(lane 2(20μg), Neuro-2a(lane 3(20μg), C6(lane 4(20μg) and Hela (lane 5(20μg) using IRE1 Antibody (HY-P83860). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human Rectal cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human liver tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human stomach tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    Immunohistochemical analysis of paraffin-embedded Human lung tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Inositol-requiring enzyme 1 (IRE1) is a bifunctional serine/threonine kinase and endoribonuclease that is a major mediator of the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. It represents a potential therapeutic target for a number of diseases associated with endoplasmic reticulum stress.

    IRE1 is the only identified ER stress sensor in yeast and essential for UPR in animals and plants. As an ER transmembrane protein, IRE1 monitors ER homeostasis through an ER luminal stress-sensing domain and triggers UPR through a cytoplasmic kinase domain and an RNase domain. Upon ER stress, IRE1 RNase is activated through conformational change, autophosphorylation, and higher order oligomerization. Mammalian IRE1 initiates diverse downstream signaling of the UPR either through unconventional splicing of the transcription factor Xbp-1 or and through posttranscriptional modifications via Regulated IRE1-Dependent Decay (RIDD) of multiple substrates.

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Widely expressed. High expression levels are observed in pancreatic tissue.

    Induction:Induced by the ER stressor tunicamycin

  • Isoforms & Post-Translational Modification

    O75460 has 2 isomers: O75460-1: 109735 Da (predicted); O75460-2: 6649 Da (predicted).
    Autophosphorylated following homodimerization. Autophosphorylation promotes activation of the endoribonuclease domain (PubMed:12637535, PubMed:21317875, PubMed:28128204, PubMed:9637683). In response to ER stress, phosphorylated at Ser-724, Ser-729 and possibly Ser-726; phosphorylation promotes oligomerization and endoribonuclease activity (PubMed:30118681). Dephosphorylated at Ser-724, Ser-729 and possibly Ser-726 by RPAP2 to abort failed ER-stress adaptation and trigger apoptosis (PubMed:30118681). Phosphorylated at Ser-724; in response to the ER stressor tunicamycin (PubMed:36739529);ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities

  • Subunit

    Monomer (PubMed:16973740, PubMed:29198525). Homodimer; disulfide-linked; homodimerization takes place in response to endoplasmic reticulum stress and promotes activation of the kinase and endoribonuclease activities (PubMed:12637535, PubMed:16973740, PubMed:21317875, PubMed:24508390, PubMed:30118681). Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges (PubMed:12637535). Interacts (via the luminal region) with DNAJB9/ERdj4; interaction takes place in unstressed cells and promotes recruitment of HSPA5/BiP (PubMed:29198525). Interacts (via the luminal region) with HSPA5/BiP; HSPA5/BiP is a negative regulator of the unfolded protein response (UPR) that prevents homodimerization of ERN1/IRE1 and subsequent activation of the protein (PubMed:12637535, PubMed:29198525, PubMed:36739529). Interaction with HSPA5 also competitively inhibits ERN1 interaction with MANF (PubMed:36739529). Interacts with PDIA6, a negative regulator of the UPR; the interaction is direct and disrupts homodimerization (PubMed:24508390). Interacts with DAB2IP (via PH domain); the interaction occurs in a endoplasmic reticulum stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1/IRE1 (By similarity). Interacts with TAOK3 and TRAF2 (PubMed:11278723). Interacts with RNF13 (PubMed:23378536). Interacts with LACC1 (PubMed:31875558). Interacts (when unphosphorylated) with DDRGK1; interaction is dependent on UFM1 and takes place in response to endoplasmic reticulum stress, regulating ERN1/IRE1-alpha stability (PubMed:28128204). Interacts (via N-terminus) with P4HB/PDIA1; the interaction is enhanced by phosphorylation of P4HB by FAM20C in response to endoplasmic reticulum stress and results in attenuation of ERN1 activity (PubMed:32149426). Interacts with TMBIM6; this interaction inhibits ERN1 activity (PubMed:19328063). Interacts (via luminal domain) with MANF (via C-terminus); the interaction is decreased in the presence of increasing concentrations of Ca(2+) (PubMed:36739529)

  • SwissProt ID

    O75460

  • Gene ID
    2081 [NCBI]
  • Synonyms

    IRE1; IRE1P; IRE1a; hIRE1p; FLJ30999; MGC163277; MGC163279; ERN1

IRE1 Antibody (YA3557) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg), Neuro-2a(lane 3(20μg), C6(lane 4(20μg) and Hela (lane 5(20μg) using IRE1 Antibody (HY-P83860). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human Rectal cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human stomach tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human lung tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    1/16
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • IRE1 Antibody (YA3557) Product Image
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293(lane 2(20μg), Neuro-2a(lane 3(20μg), C6(lane 4(20μg) and Hela (lane 5(20μg) using IRE1 Antibody (HY-P83860). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human Rectal cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human liver tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human stomach tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Human lung tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • Experimental Validation Results for IRE1 Antibody (YA3557)
  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293(lane 2(20μg), Neuro-2a(lane 3(20μg), C6(lane 4(20μg) and Hela (lane 5(20μg) using IRE1 Antibody (HY-P83860). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human Rectal cancer tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human liver tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Human lung tissue using IRE1 Antibody (HY-P83860, 1/500) . The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (PH 6.0)for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IRE1 Antibody (YA3557) Product Image WB
  • IRE1 Antibody (YA3557) Product Image IHC-P
  • IRE1 Antibody (YA3557) Product Image IHC-P
  • IRE1 Antibody (YA3557) Product Image IHC-P
  • IRE1 Antibody (YA3557) Product Image IHC-P
  • IRE1 Antibody (YA3557) Product Image IHC-P
  • IRE1 Antibody (YA3557) Product Image IHC-P