NXF1 Antibody (YA1468)(PBS only)

Cat. No.: HY-P81723A: Data Sheet User Guide for Antibodies
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NXF1 Antibody (YA1468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NXF1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

NXF1 Antibody (YA1468) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NXF1.

Host

Rabbit

분자량

Predicted band size: 70 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9UBU9

Gene ID

10482 [NCBI]

Immunogen

A synthetic peptide of human NXF1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
FC FC: Flow Cytometry	1:20-1:50
IP IP: Immunoprecipitation	1:10-1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway) (PubMed:10924507). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex (PubMed:18364396, PubMed:19165146, PubMed:9660949). ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export (PubMed:18364396, PubMed:19165146, PubMed:9660949). Also involved in nuclear export of m6A-containing mRNAs: interaction between SRSF3 and YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244)

Subcellular Localization：Nucleus; Nucleus, nucleoplasm; Nucleus speckle; Nucleus, nuclear pore complex; Nucleus envelope; Cytoplasm; Cytoplasm, Stress granule

Expression：
Tissue_specificity:Expressed ubiquitously

Isoforms & Post-Translational Modification：Q9UBU9 has 2 isomers: Q9UBU9-1: 70182 Da (predicted); Q9UBU9-2: 40476 Da (predicted).

Subunit：Heterodimer (via NTF2 domain) with NXT1 (PubMed:11583626). The formation of NXF1-NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export (PubMed:11583626). Forms a complex with RANBP2/NUP358, NXT1 and RANGAP1 (PubMed:14729961). Associates with the exon junction complex (EJC) and with the transcription/export (TREX) complex (PubMed:11707413, PubMed:22893130). Found in a mRNA complex with UPF3A and UPF3B (PubMed:11546873). Found in a post-splicing complex with RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1 (PubMed:11546874). Interacts (via N-terminus) with DHX9 (via N-terminus); this interaction is direct and negatively regulates NXF1-mediated nuclear export of constitutive transport element (CTE)-containing cellular mRNAs (PubMed:10924507). Interacts with ALYREF/THOC4 (PubMed:11707413, PubMed:14730019, PubMed:19165146, PubMed:23299939). Interacts with FYTTD1/UIF (PubMed:19836239). Interacts with EIF4A3 (PubMed:14730019). Interacts with NUP42 (PubMed:10228171). Interacts with THOC5 (PubMed:19165146, PubMed:23299939). Interacts with CHTOP (PubMed:23299939, PubMed:23826332). Interacts with FRG1 (via N-terminus) (PubMed:21699900). Interacts with LUZP4 (PubMed:25662211). Interacts with FMR1; the interaction occurs in a mRNA-dependent and polyribosomes-independent manner in the nucleus (PubMed:18936162). Interacts with CPSF6 (via N-terminus); this interaction is direct (PubMed:19864460). Interacts with RBM15 (PubMed:17001072). Interacts with RBM15B (PubMed:19586903). Interacts with MCM3AP isoform GANP; this interaction is not mediated by RNA (PubMed:20005110). Interacts with DDX3X (via C-terminus); this interaction may be partly involved in DDX3X nuclear export and in NXF1 localization to stress granules (PubMed:18596238). Interacts with PABPC1/PABP1 (PubMed:18596238)

Research Field

Tags & Cell Markers

Synonyms

DmNXF1; MEX67; Mex67p; Mvb1; NXF1; Sbr; TAP

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/u-251-cell.html [Content Brief]