Cat. No.: HY-P80249

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nNOS Antibody (YA263)

(Synonyms: Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1)

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Based on 1 publication(s) in Google Scholar

nNOS Antibody (YA263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to nNOS.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, IHC-P, ICC/IF, IP
Reactivity :
Human, Mouse, Rat

Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Conjugation:
Non-conjugated

ALL WB IHC-P ICC

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Publications (1)
Verification Images
Background
Documentation
References
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Classifications
Bioz

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	IHC-P IHC-P: Immunohistochemistry-Paraffin	IP IP: Immunoprecipitation
Dilution Ratio	1:1000-1:2000	1:50-1:200	1:50-1:200	Use at an assay dependent concentration.

Product Details

Description

nNOS Antibody (YA263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to nNOS.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 161 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 161 kDa

Species Reactivity Database

Entrez Gene: 4842 Human ; 18125 Mouse ; 24598 Rat

SwissProt: P29475 Human ; Q9Z0J4 Mouse ; P29476 Rat

OMIM: 163731 Human

Immunogen

Synthetic peptide corresponding to Human nNOS.AA range:1398-1434.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102379

Product Properties

Appearance

Solution
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Publications (1)

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Journal Impact Factor

Neurogastroenterol Motil

Berberine Improves Depression-Like Behaviors and Gastrointestinal Dysfunction in 6-Hydroxydopamine-Induced Parkinson's Disease Rats. [Abstract]

2025 Jul;37(7):e70033. PMID: 40145348

Verification Images

Western blot analysis of extracts from Hela (lane2(20μg), Rat brain tissue (lane3(20μg), Mouse brain tissue (lane4(20μg) and A549 (lane5(20μg) using nNOS Antibody (HY-P80249). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human glioma tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80249, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human brain tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80249, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human colon tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80249, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80249, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using nNOS Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of HepG2 cells labeling nNOS with nNOS antibody (HY-P80249) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with nNOS antibody (HY-P80249) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HepG2 cells labeling nNOS with nNOS antibody (HY-P80249) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with nNOS antibody (HY-P80249) at 1/100 dilution in BSA for Immunol Staining at 4℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function

Neuronal nitric oxide synthase (nNOS, NOS1) is a constitutively expressed nitric oxide synthase isoform that catalyzes nitric oxide (NO) production from L-arginine and functions as a major signaling enzyme in the central and peripheral nervous systems^[1]^[2]. Mechanistically, nNOS-derived NO participates in synaptic plasticity, long-term potentiation, and neuron-to-neuron communication through NO-dependent signaling pathways, thereby contributing to learning, memory, and neural network regulation^[2]^[3]. Beyond the nervous system, nNOS is also expressed in vascular tissues and contributes to cardiovascular homeostasis, vascular relaxation, and blood pressure regulation, indicating broader physiological functions than originally recognized^[4]. In disease-related contexts, dysregulated nNOS activity has been implicated in ischemic brain injury, neurodegenerative disorders, demyelination, and other conditions associated with excessive nitric oxide and reactive nitrogen species production^[3]^[5]. Compared with the related nitric oxide synthase isoforms, eNOS (NOS3) primarily regulates endothelial vascular function, whereas iNOS (NOS2) is inducible during inflammatory responses and generates substantially larger amounts of NO; in contrast, nNOS is calcium/calmodulin-dependent and mainly mediates physiological signaling processes^[1]^[2]^[4]. For experimental applications, selective nNOS inhibitors such as 7-nitroindazole (7-NI) have been widely used to investigate nNOS-specific functions and have demonstrated neuroprotective effects in animal models of ischemic and neurotoxic injury, making them valuable pharmacological tools for mechanistic studies^[6]^[7].
Subcellular Localization

Cell membrane, sarcolemma; Peripheral membrane protein; Cell projection, dendritic spine
Expression

Tissue_specificity:Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland
Subunit

Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1.
SwissProt ID

P29475
Gene ID

4842 [NCBI]
Synonyms

Nitric oxide synthase 1, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1, N-NOS, nNOS, bNOS, NOS1
Research Field

Immunology