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Hsp110 Antibody (YA2613)

(Synonyms: Antigen NY-CO-25; Heat shock protein 105 kDa; HS105; HSP105; HSP105A; HSP105B; HSP110; HSPH1)
Customer Review

Based on 1 Customer Validation

Hsp110 Antibody (YA2613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp110.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Hsp110 Antibody (YA2613) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50

Product Details

Description

Hsp110 Antibody (YA2613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp110.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 105 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 97 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Hsp105 aa51-99/858.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104961

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Western blot analysis of extracts from HepG2 (lane 2(20μg)), MCF7 (lane 3(20μg)), Hela (lane 3(20μg)), 3T3 (lane 3(20μg)) and PC-12 (lane 5(20μg)) using HSP110 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human pancreas using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Hsp110 acts as a nucleotide-exchange factor (NEF) for chaperone proteins HSPA1A and HSPA1B, promoting the release of ADP from HSPA1A/B thereby triggering client/substrate protein release. Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities[1].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells

  • Isoforms & Post-Translational Modification

    Q92598 has 4 isomers: Q92598-1: 96865 Da (predicted); Q92598-2: 92116 Da (predicted); Q92598-3: 92255 Da (predicted); Q92598-4: 97460 Da (predicted).
    Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity

  • Subunit

    Interacts with HSPA8/HSC70 (By similarity). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD) (PubMed:24318877)

  • SwissProt ID

    Q92598

  • Gene ID
    10808 [NCBI]
  • Synonyms

    Antigen NY-CO-25; Heat shock protein 105 kDa; HS105; HSP105; HSP105A; HSP105B; HSP110; HSPH1

  • Research Field

    Signal Transduction

Hsp110 Antibody (YA2613) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg)), MCF7 (lane 3(20μg)), Hela (lane 3(20μg)), 3T3 (lane 3(20μg)) and PC-12 (lane 5(20μg)) using HSP110 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    1/16
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Hsp110 Antibody (YA2613) Product Image
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 2(20μg)), MCF7 (lane 3(20μg)), Hela (lane 3(20μg)), 3T3 (lane 3(20μg)) and PC-12 (lane 5(20μg)) using HSP110 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Experimental Validation Results for Hsp110 Antibody (YA2613)
  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 2(20μg)), MCF7 (lane 3(20μg)), Hela (lane 3(20μg)), 3T3 (lane 3(20μg)) and PC-12 (lane 5(20μg)) using HSP110 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue using Hsp110 Antibody (HY-P82868, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Hsp110 Antibody (YA2613) Product Image WB
  • Hsp110 Antibody (YA2613) Product Image IHC-P
  • Hsp110 Antibody (YA2613) Product Image IHC-P
  • Hsp110 Antibody (YA2613) Product Image IHC-P
  • Hsp110 Antibody (YA2613) Product Image IHC-P
  • Hsp110 Antibody (YA2613) Product Image IHC-P
  • Hsp110 Antibody (YA2613) Product Image IHC-P