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Bcl2 A1 Antibody (YA2743)

(Synonyms: BCL2A1; ACC-1; ACC-2; Bcl-2-like protein 5; Bcl-2-related protein A1; BCL2-related protein A1; BFL1; Bcl2-L-5; BCL2L5; GRS; HBPA1; Protein BFL-1; Protein GRS)
Customer Review

Based on 1 Customer Validation

Bcl2 A1 Antibody (YA2743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl2 A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Bcl2 A1 Antibody (YA2743) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:2000 1:100-1:200 1:50-1:100

Product Details

Description

Bcl2 A1 Antibody (YA2743) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl2 A1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 27 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 20 kDa
Species Reactivity Database

Entrez Gene: 597 Human ;

SwissProt: Q16548 Human ;

Immunogen

A synthesized peptide derived from human BCL2A1 aa1-60.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 1), Raji (lane 2), Jurkat (lane 3), Mouse liver (lane 4), and Mouse kidney (lane 5) using Bcl2 A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    Bcl2 A1 is a Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection. Can inhibit apoptosis induced by serum starvation in the mammary epithelial cell line HC11

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies

    Induction:By phorbol ester and inflammatory cytokines, such as TNF or IL1B/interleukin-1 beta, but not by growth factors

  • Isoforms & Post-Translational Modification

    Q16548 has 2 isomers: Q16548-1: 20132 Da (predicted); Q16548-2: 18746 Da (predicted).

  • Subunit

    Interacts directly with BAK1, BID, BMF and BBC3 (By similarity). Interacts directly with BCL2L11/BIM. Interacts with BAX isoform Sigma. Interacts directly with PMAIP1. Interacts with RTL10/BOP. Interacts with ING4 (By similarity). Interacts with UBQLN4 (PubMed:34245648)

  • SwissProt ID

    Q16548

  • Gene ID
    597 [NCBI]
  • Synonyms

    BCL2A1; ACC-1; ACC-2; Bcl-2-like protein 5; Bcl-2-related protein A1; BCL2-related protein A1; BFL1; Bcl2-L-5; BCL2L5; GRS; HBPA1; Protein BFL-1; Protein GRS

  • Research Field

    Cell Biology

Bcl2 A1 Antibody (YA2743) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 1), Raji (lane 2), Jurkat (lane 3), Mouse liver (lane 4), and Mouse kidney (lane 5) using Bcl2 A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Bcl2 A1 Antibody (YA2743) Product Image
    1/16
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Bcl2 A1 Antibody (YA2743) Product Image
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 1), Raji (lane 2), Jurkat (lane 3), Mouse liver (lane 4), and Mouse kidney (lane 5) using Bcl2 A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Experimental Validation Results for Bcl2 A1 Antibody (YA2743)
  • Bcl2 A1 Antibody (YA2743) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from MCF-7 (lane 1), Raji (lane 2), Jurkat (lane 3), Mouse liver (lane 4), and Mouse kidney (lane 5) using Bcl2 A1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Bcl2 A1 Antibody (YA2743) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Hepatocellular Carcinoma tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Bcl2 A1 Antibody (YA2743) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Liver tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Bcl2 A1 Antibody (YA2743) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human ‌Colorectal cancer tissue using Bcl2 A1 antibody was performed. The section was pretreated using high-temperature mediated EDTA antigen retrieval buffer (pH 9.0), for 20 minutes. The tissues were incubated with primary antibody (HY-P82998, 1:100 dilution) at room temperature for 20 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Bcl2 A1 Antibody (YA2743) Product Image WB
  • Bcl2 A1 Antibody (YA2743) Product Image IHC-P
  • Bcl2 A1 Antibody (YA2743) Product Image IHC-P
  • Bcl2 A1 Antibody (YA2743) Product Image IHC-P