PGAM5 Antibody (YA6701)

Cat. No.: HY-P87008: Data Sheet COA
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PGAM5 Antibody (YA6701) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGAM5.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

PGAM5 Antibody (YA6701) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGAM5.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 32 kDa;

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96HS1

Gene ID

192111 [NCBI]

Immunogen

Recombinant protein within human PGAM5 aa 30-289.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using PGAM5 Antibody (HY-P87008) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed:24746696, PubMed:32439975). Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1 (PubMed:32439975). Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation (PubMed:37498743). Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3 (PubMed:24746696). Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2 (PubMed:18387606). Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria (PubMed:22265414)

Subcellular Localization：Mitochondrion outer membrane,Mitochondrion inner membrane

Isoforms & Post-Translational Modification：Q96HS1 has two isomers: Q96HS1-1: 32004 Da (predicted); Q96HS1-2: 28020 Da (predicted).
Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions丨Proteolytically cleaved by PARL in response to loss of mitochondrial membrane potential

Subunit：Dimer

RRID

AB_3719246

Synonyms

Bcl-XL-binding protein v68 antibody; BXLBv68 antibody; MGC5352 antibody; mitochondrial antibody; PGAM5 antibody; PGAM5_HUMAN antibody; Phosphoglycerate mutase family member 5 antibody; Serine/threonine protein phosphatase PGAM5 mitochondrial antibody; Serine/threonine-protein phosphatase PGAM5 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)