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PGAM5 Antibody (YA6701)

(Synonyms: Bcl-XL-binding protein v68 antibody; BXLBv68 antibody; MGC5352 antibody; mitochondrial antibody; PGAM5 antibody; PGAM5_HUMAN antibody; Phosphoglycerate mutase family member 5 antibody; Serine/threonine protein phosphatase PGAM5 mitochondrial antibody; Serine/threonine-protein phosphatase PGAM5 antibody; )
Customer Review

Based on 1 Customer Validation

PGAM5 Antibody (YA6701) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGAM5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PGAM5 Antibody (YA6701)
  • PGAM5 Antibody (YA6701) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:10000

Product Details

Description

PGAM5 Antibody (YA6701) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PGAM5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 32 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Immunogen

Recombinant protein within human PGAM5 aa 30-289.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719246

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGAM5 Antibody (YA6701)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using PGAM5 Antibody (HY-P87008) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PGAM5 is a Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy. Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1. Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation. Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3. Regulates anti-oxidative response by forming a tertiary complex with KEAP1 and NRF2. Regulates necroptosis by acting as a RIPK3 target and recruiting the RIPK1-RIPK3-MLKL necrosis 'attack' complex to mitochondria[1][2][3][4][5].

  • Subcellular Localization

    Mitochondrion outer membrane,Mitochondrion inner membrane

  • Isoforms & Post-Translational Modification

    Q96HS1 has two isomers: Q96HS1-1: 32004 Da (predicted); Q96HS1-2: 28020 Da (predicted).
    Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions丨Proteolytically cleaved by PARL in response to loss of mitochondrial membrane potential

  • Subunit

    Dimer

  • SwissProt ID

    Q96HS1

  • Gene ID
    192111 [NCBI]
  • Synonyms

    Bcl-XL-binding protein v68 antibody; BXLBv68 antibody; MGC5352 antibody; mitochondrial antibody; PGAM5 antibody; PGAM5_HUMAN antibody; Phosphoglycerate mutase family member 5 antibody; Serine/threonine protein phosphatase PGAM5 mitochondrial antibody; Serine/threonine-protein phosphatase PGAM5 antibody;

PGAM5 Antibody (YA6701) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using PGAM5 Antibody (HY-P87008) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PGAM5 Antibody (YA6701) Product Image
    1/16
  • Experimental Validation Results for PGAM5 Antibody (YA6701)
  • PGAM5 Antibody (YA6701) Product Image
  • Experimental Validation Results for PGAM5 Antibody (YA6701)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using PGAM5 Antibody (HY-P87008) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PGAM5 Antibody (YA6701)
  • PGAM5 Antibody (YA6701) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、A549 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and C6 (lane 4(20μg)) using PGAM5 Antibody (HY-P87008) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/10000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • PGAM5 Antibody (YA6701) Product Image WB