NELFE Antibody (YA1365)(PBS only)

Cat. No.: HY-P81620A: Data Sheet User Guide for Antibodies
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NELFE Antibody (YA1365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFE.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NELFE Antibody (YA1365) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NELFE.

Host

Rabbit

Molecular Weight

Predicted band size: 43 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P18615

Gene ID

7936 [NCBI]

Immunogen

Recombinant protein of human NELFe

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
FC FC: Flow Cytometry	1:200-1:500
IP IP: Immunoprecipitation	1:10-1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401, PubMed:27256882). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed:11940650, PubMed:12612062, PubMed:27256882). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (PubMed:18303858, PubMed:27256882, PubMed:27282391); (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas

Isoforms & Post-Translational Modification：P18615 has 3 isomers: P18615-1: 43240 Da (predicted); P18615-3: 43925 Da (predicted); P18615-4: 40216 Da (predicted).
Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin;Sumoylated;Poly-ADP-ribosylated by PARP1, thereby preventing RNA-binding and relieving transcription pausing

Subunit：The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE (PubMed:10199401, PubMed:27282391). Interacts with NELFB (By similarity)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

RD; RDP; RDBP; D6S45; NELF-E

Documentation

User Guide for Antibodies (1077 KB)