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OBFC1 Antibody (YA7820)

(Synonyms: AAF-44; AAF44; bA541N10.2; RPA-32; STN1)
Customer Review

Based on 1 Customer Validation

OBFC1 Antibody (YA7820) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OBFC1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • OBFC1 Antibody (YA7820) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000-3000 1:100-250 1:100

Product Details

Description

OBFC1 Antibody (YA7820) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OBFC1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Calculated Molecular Weight Predicted band size: 41.9 kDa
Immunogen

Full length human recombinant protein of human OBFC1 produced in HEK293T cell.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    Western blot analysis of extracts from Hela (lane2(20μg), HEK293T (lane3(20μg), MCF-7 (lane4(20μg) and THP-1 (lane5(20μg) using OBFC1 Antibody (HY-P88136). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    Immunocytochemistry analysis of A431 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    Immunocytochemistry analysis of HePG2 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    OBFC1 component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation. However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres. Promotes general replication start following replication-fork stalling implicating new origin firing. May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication |Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites[1][2][3][4][5][6].

  • Subcellular Localization

    Nucleus,Chromosome, telomere

  • Isoforms & Post-Translational Modification

    Q9H668: 368 amino acids, molecular weight 42119 Da.

  • Subunit

    Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1; in the complex interacts directly with TEN1 and CTC1

  • SwissProt ID

    Q9H668

  • Synonyms

    AAF-44; AAF44; bA541N10.2; RPA-32; STN1

OBFC1 Antibody (YA7820) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), HEK293T (lane3(20μg), MCF-7 (lane4(20μg) and THP-1 (lane5(20μg) using OBFC1 Antibody (HY-P88136). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OBFC1 Antibody (YA7820) Product Image
    1/16
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • OBFC1 Antibody (YA7820) Product Image
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), HEK293T (lane3(20μg), MCF-7 (lane4(20μg) and THP-1 (lane5(20μg) using OBFC1 Antibody (HY-P88136). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HePG2 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • Experimental Validation Results for OBFC1 Antibody (YA7820)
  • OBFC1 Antibody (YA7820) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), HEK293T (lane3(20μg), MCF-7 (lane4(20μg) and THP-1 (lane5(20μg) using OBFC1 Antibody (HY-P88136). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • OBFC1 Antibody (YA7820) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OBFC1 Antibody (YA7820) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HePG2 cells labeling OBFC1 with OBFC1 Antibody (HY-P88136) at 1/100 dilution . Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with OBFC1 Antibody (HY-P88136) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • OBFC1 Antibody (YA7820) Product Image WB
  • OBFC1 Antibody (YA7820) Product Image ICC
  • OBFC1 Antibody (YA7820) Product Image ICC