OBFC1 Antibody (YA7820)

Cat. No.: HY-P88136: Data Sheet COA
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OBFC1 Antibody (YA7820) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OBFC1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

OBFC1 Antibody (YA7820) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to OBFC1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 41.9 kDa

Species Reactivity

Human

SwissProt ID

Q9H668

Immunogen

Full length human recombinant protein of human OBFC1 produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-3000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-250
FC FC: Flow Cytometry	1:100

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane2(20μg), HEK293T (lane3(20μg), MCF-7 (lane4(20μg) and THP-1 (lane5(20μg) using OBFC1 Antibody (HY-P88136). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis; it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha (PubMed:22763445, PubMed:22964711). The CST complex facilitates recovery from many forms of exogenous DNA damage; seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins (PubMed:25483097). Required for efficicient replication of the duplex region of the telomere. Promotes efficient replication of lagging-strand telomeres (PubMed:22863775, PubMed:22964711). Promotes general replication start following replication-fork stalling implicating new origin firing (PubMed:22863775). May be in involved in C-strand fill-in during late S/G2 phase independent of its role in telomere duplex replication (PubMed:23142664)|Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites

Subcellular Localization：Nucleus,Chromosome, telomere

Isoforms & Post-Translational Modification：Q9H668: 368 amino acids, molecular weight 42119 Da.

Subunit：Component of the CST complex, composed of TEN1/C17orf106, CTC1/C17orf68 and STN1; in the complex interacts directly with TEN1 and CTC1

Synonyms

AAF-44; AAF44; bA541N10.2; RPA-32; STN1

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)