Copy Link Print Information

Phospho-ULK1 (Ser556) Antibody (YA908)

(Synonyms: ATG1; ATG1A; hATG1; ULK1; UNC51; )
Customer Review

Based on 1 Customer Validation

Phospho-ULK1 (Ser556) Antibody (YA908) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ULK1 (Ser556).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Phospho-ULK1 (Ser556) Antibody (YA908) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Phospho-ULK1 (Ser556) Antibody (YA908) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-ULK1 (Ser556).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Calculated Molecular Weight Calculated MW:113kDa; Observed MW:130kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Phospho-ULK1 (S556)

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3103047

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    ULK1 is a serine/threonine kinase and a key initiator of mammalian autophagy, functioning near the apex of a nutrient-sensitive regulatory pathway[1]. Mechanistically, AMPK binds ULK1 and supports ULK1-mediated autophagy, while mTORC1 negatively regulates the ULK1 autophagic complex through phosphorylation-dependent control[2][3]. During starvation, ULK1 links cellular energy sensing to downstream autophagy machinery, including ATG13, FIP200/RB1CC1, ATG101, ATG14L, VPS34, and phagophore formation[3][4]. In disease models, altered ULK1 signaling has been connected with prostate cancer progression, prion infection, clear cell renal cell carcinoma, cardiomyocyte oxidative injury, and pathogen-related autophagy[5][6][7][8][9]. Compared with ULK2, ULK1 shows strong functional overlap in nutrient-starvation autophagy, because combined ULK1/ULK2 loss disrupts autophagy activation more clearly than single loss[10]. Compared with ULK3, ULK1 remains part of the canonical mTOR-AMPK autophagy axis, whereas ULK3 can support mTOR-insensitive autophagic flux in BIN1-deficient hippocampal neurons[11]. For experimental applications, ULK1 pathway modulation can be studied with AMPK activators, mTOR inhibitors, deubiquitinase inhibitor WP1130, and pathway inhibitors targeting CAMKK2-AMPK-ULK1 signaling[2][5][12].

  • Subcellular Localization

    Cytoplasm, cytosol; Preautophagosomal structure

  • Expression


    Tissue_specificity:Widely expressed. Detectable in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidneys, and lungs.

  • Subunit

    Interacts with GABARAP and GABARAPL2 (PubMed:11146101). Interacts (via C-terminus) with ATG13 (PubMed:18936157). Part of a complex consisting of ATG13, ATG101, ULK1 and RB1CC1 (PubMed:19287211). Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation (PubMed:19211835, PubMed:21795849). Interacts with FEZ1; SCOC interferes with FEZ1-binding (PubMed:22354037). Interacts with TBC1D14 (PubMed:22613832). Interacts (phosphorylated form) with TRIM5 (PubMed:25127057). When phosphorylated at Ser-317, interacts with MEFV and BECN1 simultaneously (PubMed:26347139). Interacts with TRIM21 and IRF3, in the presence of TRIM21 (PubMed:26347139). Interacts with SESN2 (PubMed:25040165). Interacts with SQSTM1 (PubMed:25040165). Interacts with C9orf72 (PubMed:27334615). Interacts with WDR45 (PubMed:28561066). Interacts with ATG13; this interaction is increased in the absence of TMEM39A (PubMed:31806350). Interacts with WIPI2 (PubMed:28890335). Interacts with ATP2A2 (PubMed:28890335). Interacts with AMBRA1 (PubMed:25438055, PubMed:31123703). Interacts with IRGM; promoting the coassembly of ULK1 and BECN1 (PubMed:25891078)

  • SwissProt ID

    O75385

  • Gene ID
    8408 [NCBI]
  • Synonyms

    ATG1; ATG1A; hATG1; ULK1; UNC51;

  • Research Field

    Signal Transduction

References

[1]. Chan EY. Regulation and function of uncoordinated-51 like kinase proteins. Antioxid Redox Signal. 2012 Sep 1;17(5):775-85. doi: 10.1089/ars.2011.4396. Epub 2012 Jan 4. PMID: 22074133. et al. Regulation and function of uncoordinated-51 like kinase proteins. Antioxid Redox Signal. 2012 Sep 1;17(5):775-85. [Content Brief]

[2]. Lee JW, et al. The association of AMPK with ULK1 regulates autophagy. PLoS One. 2010 Nov 3;5(11):e15394. [Content Brief]

[3]. Roach PJ. AMPK -> ULK1 -> autophagy. Mol Cell Biol. 2011 Aug;31(15):3082-4. doi: 10.1128/MCB.05565-11. Epub 2011 May 31. PMID: 21628530; PMCID: PMC3147606. et al. AMPK -> ULK1 -> autophagy. Mol Cell Biol. 2011 Aug;31(15):3082-4. [Content Brief]

[4]. Pyo KE, et al. ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation. Cell Rep. 2018 Dec 4;25(10):2878-2890.e4. [Content Brief]

[5]. Lin C, et al. Inhibition of CAMKK2 impairs autophagy and castration-resistant prostate cancer via suppression of AMPK-ULK1 signaling. Oncogene. 2021 Mar;40(9):1690-1705. [Content Brief]

[6]. Fan XY, et al. Activation of the AMPK-ULK1 pathway plays an important role in autophagy during prion infection. Sci Rep. 2015 Oct 1;5:14728. [Content Brief]

[7]. Radovanovic M, et al. Role of AMPK/mTOR-independent autophagy in clear cell renal cell carcinoma. J Investig Med. 2020 Dec;68(8):1386-1393. [Content Brief]

[8]. Xie C, et al. A novel danshensu/tetramethypyrazine derivative attenuates oxidative stress-induced autophagy injury via the AMPK-mTOR-Ulk1 signaling pathway in cardiomyocytes. Exp Ther Med. 2021 Feb;21(2):118. [Content Brief]

[9]. Yu Y, et al. Guanylate-Binding protein 2b regulates the AMPK/mTOR/ULK1 signalling pathway to induce autophagy during Mycobacterium bovis infection. Virulence. 2022 Dec;13(1):875-889. [Content Brief]

[10]. McAlpine F, et al. Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy. 2013 Mar;9(3):361-73. [Content Brief]

[11]. Jin Y, et al. BIN1 deficiency enhances ULK3-dependent autophagic flux and reduces dendritic size in mouse hippocampal neurons. Autophagy. 2025 Jan;21(1):223-242. [Content Brief]

[12]. Drießen S, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11(9):1458-70. [Content Brief]

View More

Phospho-ULK1 (Ser556) Antibody (YA908) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Phospho-ULK1 (Ser556) Antibody (YA908) Product Image
    1/16
  • Phospho-ULK1 (Ser556) Antibody (YA908) Product Image