PPAR gamma Antibody (YA5394)

Cat. No.: HY-P85702: Data Sheet User Guide for Antibodies
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PPAR gamma Antibody (YA5394) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PPAR gamma.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PPAR gamma Antibody (YA5394) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PPAR gamma.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 53-57 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P37231

Gene ID

5468 [NCBI]

Immunogen

specific for an epitope mapping between amino acids 486-505 at the C-terminus of PPARγ of human origin (identical to corresponding mouse sequence)

Application &
Dilution Ratio

Application	Dilution Ratio
IHC	1:50-200
WB WB: Western Blot	1:1000-2000
IP IP: Immunoprecipitation	1:1000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Store at 4℃.

Shipping

Shipping with blue ice.

Background

Function：Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity); (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is expressed most highly in adipose tissue. It is expressed at lower levels in skeletal muscle, spleen, heart, and liver. It can also be detected in the placenta, lungs, and ovaries.

Induction: (Microbial infection) Expression increases when incubated with M.tuberculosis or its lipoprotein LpqH; induction is TLR2-dependent (at protein level)

Isoforms & Post-Translational Modification：P37231 has 3 isomers: P37231-1: 57620 Da (predicted); P37231-2: 54681 Da (predicted); P37231-3: 21580 Da (predicted).
O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes;Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity);Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9; leading to proteasomal degradation (By similarity). Ubiquitinated at Lys-252 by TRIM55 leading to proteasomal degradation (PubMed:36737649). Ubiquitinated by E3 ubiquitin-protein ligase STUB1/CHIP; leading to proteasomal degradation (By similarity)

Subunit：Interacts with FOXO1 (acetylated form) (By similarity). Heterodimer with other nuclear receptors, such as RXRA. The heterodimer with the retinoic acid receptor RXRA is called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 and NCOA2 LXXLL motifs. Interacts with ASXL1, ASXL2, DNTTIP2, FAM120B, MAP2K1/MEK1, NR0B2, PDPK1, PRDM16, PRMT2 and TGFB1I1. Interacts (when activated by agonist) with PPP5C. Interacts with HELZ2 and THRAP3; the interaction stimulates the transcriptional activity of PPARG. Interacts with PER2, the interaction is ligand dependent and blocks PPARG recruitment to target promoters. Interacts with NOCT. Interacts with ACTN4. Interacts (when in the liganded conformation) with GPS2 (By similarity). Interacts with CRY1 and CRY2 in a ligand-dependent manner (By similarity). In the absence of hormonal ligand, interacts with TACC1 (PubMed:20078863). In macrophages, interacts with PAQR3 and STUB1; the interactions promote PPARG poylubiquitination and STUB1-mediated degradation (By similarity)

Synonyms

Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3;

Documentation

User Guide for Antibodies (1077 KB)