ZIP7 Antibody
(Synonyms: HKE4, RING5, SLC39A7, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4, Really interesting new gene 5 protein, Solute carrier family 39 member 7, ZIP7)ZIP7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ZIP7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 |
Product Details
ZIP7 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ZIP7.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
Synthetic peptide corresponding to the center region of human ZIP7.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ZIP7 transports Zn(2+) from the endoplasmic reticulum (ER)/Golgi apparatus to the cytosol, playing an essential role in the regulation of cytosolic zinc levels. Acts as a gatekeeper of zinc release from intracellular stores, requiring post-translational activation by phosphorylation, resulting in activation of multiple downstream pathways leading to cell growth and proliferation. Has an essential role in B cell development and is required for proper B cell receptor signaling. Plays an important role in maintaining intestinal epithelial homeostasis and skin dermis development by regulating ER function (By similarity). Controls cell signaling pathways involved in glucose metabolism in skeletal muscle (By similarity). Has a protective role against ER stress in different biological contexts. Mediates Zn(2+)-induced ferroptosis[1][2][3][4][5][6][7][8].
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Subcellular Localization
Endoplasmic reticulum membrane; Golgi apparatus, cis-Golgi network membrane
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Expression
Tissue_Specificity: Widely expressed.
Induction: Down-regulated by Zn(+2). -
Isoforms & Post-Translational Modification
ZIP7 has an amino acid length of 469, molecular weight is 50118 Da.经 Zn(2+)处理后可迅速被 CK2 磷酸化。该磷酸化是胞质中 Zn(2+)有效释放所必需的ZIP7 的氨基酸个数为 469 个，分子量为 50118 Da。Rapidly phosphorylated by CK2 following Zn(2+) treatment. This phosphorylation is required for efficient cytosolic Zn(2+) release
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Subunit
Homodimer
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SwissProt ID
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Synonyms
HKE4, RING5, SLC39A7, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4, Really interesting new gene 5 protein, Solute carrier family 39 member 7, ZIP7
Documentation
[1]. Taylor KM, et al. Structure-function analysis of HKE4, a member of the new LIV-1 subfamily of zinc transporters. Biochem J. 2004 Jan 1;377(Pt 1):131-9. [Content Brief]
[2]. Huang L, et al. The ZIP7 gene (Slc39a7) encodes a zinc transporter involved in zinc homeostasis of the Golgi apparatus. J Biol Chem. 2005 Apr 15;280(15):15456-63. [Content Brief]
[3]. Nimmanon T, et al. Phosphorylation of zinc channel ZIP7 drives MAPK, PI3K and mTOR growth and proliferation signalling. Metallomics. 2017 May 24;9(5):471-481. [Content Brief]
[4]. Woodruff G, et al. The Zinc Transporter SLC39A7 (ZIP7) Is Essential for Regulation of Cytosolic Zinc Levels. Mol Pharmacol. 2018 Sep;94(3):1092-1100. [Content Brief]
[5]. Taylor KM, et al. Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Sci Signal. 2012 Feb 7;5(210):ra11. [Content Brief]
[6]. Anzilotti C, et al. An essential role for the Zn(2+) transporter ZIP7 in B cell development. Nat Immunol. 2019 Mar;20(3):350-361. [Content Brief]
[7]. Fauster A, et al. Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Cell Death Differ. 2019 Jun;26(6):1138-1155. [Content Brief]
[8]. Chen PH, et al. Zinc transporter ZIP7 is a novel determinant of ferroptosis. Cell Death Dis. 2021 Feb 19;12(2):198. [Content Brief]
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