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NDP52 Antibody (YA6674)

(Synonyms: Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Nuclear domain 10 protein antibody; Nuclear domain 10 protein NDP52 antibody; Nuclear domain protein 52 antibody; Nuclear dot protein 52 antibody; OTTHUMP00000218344 antibody; OTTHUMP00000218345 antibody; OTTHUMP00000218346 antibody; OTTHUMP00000218347 antibody; OTTHUMP00000218349 antibody; OTTHUMP00000218351 antibody; )
Customer Review

Based on 1 Customer Validation

NDP52 Antibody (YA6674) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDP52.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NDP52 Antibody (YA6674)
  • NDP52 Antibody (YA6674) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:1000

Product Details

Description

NDP52 Antibody (YA6674) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDP52.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 52 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Immunogen

Recombinant protein within human NDP52 aa 1-150 / 446 (Q13137).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719241

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NDP52 Antibody (YA6674)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Hela (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using NDP52 Antibody (HY-P86981) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    NDP52 is a Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion[1][2][3][4].

  • Subcellular Localization

    Cytoplasm, perinuclear region,Cytoplasm, cytoskeleton,Cytoplasmic vesicle, autophagosome membrane

  • Expression


    Tissue_Specificity: Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain
    Induction: Treatment with IFNB1/IFN-beta and IFNG/IFN-gamma show an increase in number and size of CALCOCO2-specific dots and partial redistribution to the cytoplasm (PubMed:7540613). IFNG/IFN-gamma increases gene expression only slightly and IFNB does not increase expression (PubMed:9230084)

  • Isoforms & Post-Translational Modification

    Q13137 has 5 isomers: Q13137-1: 52254 Da (predicted); Q13137-2: 47340 Da (predicted); Q13137-3: 54605 Da (predicted); Q13137-4: 55188 Da (predicted); Q13137-5: 43669 Da (predicted).
    (Microbial infection) Cleaved by S

  • Subunit

    Dimer (PubMed:23511477)

  • SwissProt ID

    Q13137

  • Gene ID
    10241 [NCBI]
  • Synonyms

    Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Nuclear domain 10 protein antibody; Nuclear domain 10 protein NDP52 antibody; Nuclear domain protein 52 antibody; Nuclear dot protein 52 antibody; OTTHUMP00000218344 antibody; OTTHUMP00000218345 antibody; OTTHUMP00000218346 antibody; OTTHUMP00000218347 antibody; OTTHUMP00000218349 antibody; OTTHUMP00000218351 antibody;

NDP52 Antibody (YA6674) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Hela (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using NDP52 Antibody (HY-P86981) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NDP52 Antibody (YA6674) Product Image
    1/16
  • Experimental Validation Results for NDP52 Antibody (YA6674)
  • NDP52 Antibody (YA6674) Product Image
  • Experimental Validation Results for NDP52 Antibody (YA6674)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Hela (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using NDP52 Antibody (HY-P86981) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NDP52 Antibody (YA6674)
  • NDP52 Antibody (YA6674) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Hela (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using NDP52 Antibody (HY-P86981) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • NDP52 Antibody (YA6674) Product Image WB