NDP52 Antibody (YA6674)

Cat. No.: HY-P86981: Data Sheet COA
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NDP52 Antibody (YA6674) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDP52.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

NDP52 Antibody (YA6674) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NDP52.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q13137

Gene ID

10241 [NCBI]

Immunogen

Recombinant protein within human NDP52 aa 1-150 / 446 (Q13137).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、Hela (lane 2(20μg)) 、RAW264.7 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using NDP52 Antibody (HY-P86981) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994)

Subcellular Localization：Cytoplasm, perinuclear region,Cytoplasm, cytoskeleton,Cytoplasmic vesicle, autophagosome membrane

Expression：
Tissue_Specificity: Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain
Induction: Treatment with IFNB1/IFN-beta and IFNG/IFN-gamma show an increase in number and size of CALCOCO2-specific dots and partial redistribution to the cytoplasm (PubMed:7540613). IFNG/IFN-gamma increases gene expression only slightly and IFNB does not increase expression (PubMed:9230084)

Isoforms & Post-Translational Modification：Q13137 has 5 isomers: Q13137-1: 52254 Da (predicted); Q13137-2: 47340 Da (predicted); Q13137-3: 54605 Da (predicted); Q13137-4: 55188 Da (predicted); Q13137-5: 43669 Da (predicted).
(Microbial infection) Cleaved by S

Subunit：Dimer (PubMed:23511477)

RRID

AB_3719241

Synonyms

Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Antigen nuclear dot 52 kDa protein antibody; CACO2_HUMAN antibody; Calcium binding and coiled coil domain 2 antibody; Calcium binding and coiled coil domain containing protein 2 antibody; Calcium-binding and coiled-coil domain-containing protein 2 antibody; CALCOCO 2 antibody; CALCOCO2 antibody; MGC17318 antibody; NDP52 antibody; Nuclear domain 10 protein 52 antibody; Nuclear domain 10 protein antibody; Nuclear domain 10 protein NDP52 antibody; Nuclear domain protein 52 antibody; Nuclear dot protein 52 antibody; OTTHUMP00000218344 antibody; OTTHUMP00000218345 antibody; OTTHUMP00000218346 antibody; OTTHUMP00000218347 antibody; OTTHUMP00000218349 antibody; OTTHUMP00000218351 antibody;

Documentation

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Data Sheet (231 KB) SDS (253 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)