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PLEKHA4(N-term) Antibody

(Synonyms: Pleckstrin homology domain-containing family A member 4, PH domain-containing family A member 4, Phosphoinositol 3-phosphate-binding protein 1, PEPP-1, PLEKHA4, PEPP1)
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Based on 1 Customer Validation

PLEKHA4(N-term) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PLEKHA4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PLEKHA4(N-term) Antibody
  • PLEKHA4(N-term) Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:100-1:500 1:10-1:50

Product Details

Description

PLEKHA4(N-term) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PLEKHA4.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Immunogen

KLH conjugated synthetic peptide derived from human PLEKHA4: 21-50 aa.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PLEKHA4(N-term) Antibody
    Flow cytometric analysis of 1X106 K562 cells labeling PLEKHA4(N-term) Antibody (HY-P810314, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    PLEKHA4 binds specifically to phosphatidylinositol 3-phosphate (PtdIns3P), but not to other phosphoinositides

  • Subcellular Localization

    Cytoplasm; Membrane

  • Expression


    Tissue_Specificity: Highly expressed in melanoma. Detected at low levels in heart, skeletal muscle, kidney, liver and small intestine.

  • Isoforms & Post-Translational Modification

    PLEKHA4 has 2 isoforms, Q9H4M7-1: amino acid length is 779, molecular weight is 85401 Da (predicted); Q9H4M7-2: amino acid length is 583, molecular weight is 64190 Da (predicted).

  • SwissProt ID

    Q9H4M7

  • Gene ID
    57664 [NCBI]
  • Synonyms

    Pleckstrin homology domain-containing family A member 4, PH domain-containing family A member 4, Phosphoinositol 3-phosphate-binding protein 1, PEPP-1, PLEKHA4, PEPP1

PLEKHA4(N-term) Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling PLEKHA4(N-term) Antibody (HY-P810314, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PLEKHA4(N-term) Antibody Product Image
    1/16
  • Experimental Validation Results for PLEKHA4(N-term) Antibody
  • PLEKHA4(N-term) Antibody Product Image
  • Experimental Validation Results for PLEKHA4(N-term) Antibody
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling PLEKHA4(N-term) Antibody (HY-P810314, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for PLEKHA4(N-term) Antibody
  • PLEKHA4(N-term) Antibody Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling PLEKHA4(N-term) Antibody (HY-P810314, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • PLEKHA4(N-term) Antibody Product Image FC