2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. PAX8 Antibody (YA3178)

PAX8 Antibody (YA3178) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX8.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Description

  • Références

Description

PAX8 Antibody (YA3178) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX8.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Masse moléculaire
Predicted band size: 48 kDa;
Observed band size: 48 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human PAX8 aa120-450 aa120-450/450.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
Sensitivity Endogenous Pureté Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from SKOV3 (lane 2(20μg)) using PAX8 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells
Subcellular Localization：Nucleus
Expression：
Tissue_specificity:It is expressed in the urinary system, thyroid gland, and nephroblastoma.
Isoforms & Post-Translational Modification：Q06710 has 5 isomers: Q06710-1: 48218 Da (predicted); Q06710-2: 41741 Da (predicted); Q06710-3: 43028 Da (predicted); Q06710-4: 34909 Da (predicted); Q06710-5: 31044 Da (predicted).
Subunit：Interacts with WWTR1
RRID
Database

Entrez Gene: 7849 Human

SwissProt: Q06710 Human

OMIM: 218700 Human

Research Field

Neuroscience
Synonyms
Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired domain gene 8; PAX8
Documentation
SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
Références

PAX8 Antibody (YA3178) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nom du produit:
PAX8 Antibody (YA3178)
Cat. No.:
HY-P83433
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