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PAX8 Antibody (YA3178)

(Synonyms: Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired domain gene 8; PAX8)
Customer Review

Based on 1 Customer Validation

PAX8 Antibody (YA3178) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX8.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PAX8 Antibody (YA3178)
  • PAX8 Antibody (YA3178) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

PAX8 Antibody (YA3178) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PAX8.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database

Entrez Gene: 7849 Human

SwissProt: Q06710 Human

Immunogen

A synthesized peptide derived from human PAX8 aa120-450 aa120-450/450.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105544

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PAX8 Antibody (YA3178)
    Western blot analysis of extracts from SKOV3 (lane 2(20μg)) using PAX8 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    PAX8 transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is expressed in the urinary system, thyroid gland, and nephroblastoma.

  • Isoforms & Post-Translational Modification

    Q06710 has 5 isomers: Q06710-1: 48218 Da (predicted); Q06710-2: 41741 Da (predicted); Q06710-3: 43028 Da (predicted); Q06710-4: 34909 Da (predicted); Q06710-5: 31044 Da (predicted).

  • Subunit

    Interacts with WWTR1

  • SwissProt ID

    Q06710

  • Gene ID
    7849 [NCBI]
  • Synonyms

    Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired domain gene 8; PAX8

  • Research Field

    Neuroscience

PAX8 Antibody (YA3178) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from SKOV3 (lane 2(20μg)) using PAX8 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PAX8 Antibody (YA3178) Product Image
    1/16
  • Experimental Validation Results for PAX8 Antibody (YA3178)
  • PAX8 Antibody (YA3178) Product Image
  • Experimental Validation Results for PAX8 Antibody (YA3178)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from SKOV3 (lane 2(20μg)) using PAX8 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PAX8 Antibody (YA3178)
  • PAX8 Antibody (YA3178) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from SKOV3 (lane 2(20μg)) using PAX8 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PAX8 Antibody (YA3178) Product Image WB