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Synaptophysin Antibody (YA043)

Customer Review

Based on 1 Customer Validation

Synaptophysin Antibody (YA043) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Synaptophysin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Synaptophysin Antibody (YA043) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:1000

Product Details

Description

Synaptophysin Antibody (YA043) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Synaptophysin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 34 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 34 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within Human Synaptophysin aa 250 to the C-terminus.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102894

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    Western blot analysis of extracts from HEK293T (lane 2(20μg) , HEK293T (lane 3(40μg),using Synaptophysin Antibody (HY-P80354). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity)

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein; Synapse, synaptosome

  • Expression


    Tissue_specificity:This gene is expressed in the brain, primarily in the hippocampus, dentate gyrus neural stack (expression is higher in the outer half of the molecular layer than in the inner half) , and CA4 and CA3 neural stacks (PubMed: 8838578) . It is also expressed in the putamen (protein level) (PubMed: 17296554) .

  • Isoforms & Post-Translational Modification

    P08247 has 2 isomers: P08247-1: 33845 Da (predicted); P08247-2: 20757 Da (predicted).
    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation;Phosphorylated by SRC

  • Subunit

    Homohexamer or homotetramer. Interacts with SRCIN1 (PubMed:18662323). Interacts with VAMP2; the interaction is inhibited by interaction of VAPM2 with SEPT8 (By similarity)

  • SwissProt ID

    P08247

  • Gene ID
    6855 [NCBI]
  • Research Field

    Neuroscience

Synaptophysin Antibody (YA043) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg) , HEK293T (lane 3(40μg),using Synaptophysin Antibody (HY-P80354). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Synaptophysin Antibody (YA043) Product Image
    1/16
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Synaptophysin Antibody (YA043) Product Image
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293T (lane 2(20μg) , HEK293T (lane 3(40μg),using Synaptophysin Antibody (HY-P80354). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Experimental Validation Results for Synaptophysin Antibody (YA043)
  • Synaptophysin Antibody (YA043) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293T (lane 2(20μg) , HEK293T (lane 3(40μg),using Synaptophysin Antibody (HY-P80354). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Synaptophysin Antibody (YA043) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Synaptophysin Antibody (YA043) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue using Synaptophysin Antibody (YA043). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80354, 1:1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Synaptophysin Antibody (YA043) Product Image WB
  • Synaptophysin Antibody (YA043) Product Image IHC-P
  • Synaptophysin Antibody (YA043) Product Image IHC-P