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Phosphoserine Antibody (YA5756)

Customer Review

Based on 1 Customer Validation

Phosphoserine Antibody (YA5756) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phosphoserine.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB, IHC-P, ICC/IF, IP

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phosphoserine Antibody (YA5756)
  • Phosphoserine Antibody (YA5756) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:2000 1:100-1:300 1:40000 1:50-200 1:50-200

Product Details

Description

Phosphoserine Antibody (YA5756) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phosphoserine.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 25-300 kDa；
Immunogen

Purified Protein

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Phosphorylated

RRID

AB_3719069

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phosphoserine Antibody (YA5756)
    Western blot analysis of extracts from Hela (lane2, 20μg), HeLa treated with 100nM calyculin A for 30min (lane3, 20μg) using Phosphoserine Antibody (HY-P86064). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Phosphoserine is a Phosphoserine is an enzyme that catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.

Phosphoserine Antibody (YA5756) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), HeLa treated with 100nM calyculin A for 30min (lane3, 20μg) using Phosphoserine Antibody (HY-P86064). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Phosphoserine Antibody (YA5756) Product Image
    1/16
  • Experimental Validation Results for Phosphoserine Antibody (YA5756)
  • Phosphoserine Antibody (YA5756) Product Image
  • Experimental Validation Results for Phosphoserine Antibody (YA5756)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), HeLa treated with 100nM calyculin A for 30min (lane3, 20μg) using Phosphoserine Antibody (HY-P86064). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phosphoserine Antibody (YA5756)
  • Phosphoserine Antibody (YA5756) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), HeLa treated with 100nM calyculin A for 30min (lane3, 20μg) using Phosphoserine Antibody (HY-P86064). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Phosphoserine Antibody (YA5756) Product Image WB