Thioredoxin Antibody (YA5080)(PBS only)

Cat. No.: HY-P85388A: Data Sheet User Guide for Antibodies
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Thioredoxin Antibody (YA5080) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Thioredoxin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Thioredoxin Antibody (YA5080) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Thioredoxin.

Host

Mouse

Species Reactivity

Species independent

SwissProt ID

P10599

Gene ID

7295 [NCBI]

Immunogen

Recombinant human Thioredoxin. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	affinity purified by Protein G	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029); ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)

Subcellular Localization：Nucleus; Cytoplasm; Secreted

Expression：
Induction:Up-regulated by ionizing radiation

Isoforms & Post-Translational Modification：P10599 has 2 isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins;In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation

Subunit：Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469). Interacts with TXNIP through the redox-active site (PubMed:17260951). Interacts with MAP3K5 and CASP3 (PubMed:15246877). In case of infection, interacts with S.typhimurium protein slrP (PubMed:19690162). Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner (PubMed:9108029)

Synonyms

TXN; TRDX; TRX; TRX1; Thioredoxin; Trx; ATL-derived factor; ADF; Surface-associated sulphydryl protein; SASP

Documentation

User Guide for Antibodies (1077 KB)