RAB18 Antibody
(Synonyms: Ras-related protein Rab-18, RAB18)RAB18 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB18.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
RAB18 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to RAB18.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 23; 26 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 22 kDa
Synthetic peptide corresponding to the center region of human RAB18.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
RAB18 is a The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB18 is required for the localization of ZFYVE1 to lipid droplets and for its function in mediating the formation of endoplasmic reticulum-lipid droplets (ER-LD) contacts. Also required for maintaining endoplasmic reticulum structure. Plays a role in apical endocytosis/recycling (By similarity). Plays a key role in eye and brain development and neurodegeneration[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane; Golgi apparatus, cis-Golgi network membrane; Lipid droplet; Apical cell membrane
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
RAB18 has 3 isoforms, Q9NP72-1: amino acid length is 206, molecular weight is 22977 Da (predicted); Q9NP72-2: amino acid length is 235, molecular weight is 26410 Da (predicted); Q9NP72-3: amino acid length is 142, molecular weight is 15508 Da (predicted).RAB18 存在 3 个异构体，Q9NP72-1：氨基酸个数为 206 个，分子量为 22977 Da (预测)；Q9NP72-2：氨基酸个数为 235 个，分子量为 26410 Da (预测)；Q9NP72-3：氨基酸个数为 142 个，分子量为 15508 Da (预测)。
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Subunit
Interacts (in GTP-bound form) with ZFYVE1.
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SwissProt ID
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Synonyms
Ras-related protein Rab-18, RAB18
Documentation
[1]. Gerondopoulos A, et al. Rab18 and a Rab18 GEF complex are required for normal ER structure. J Cell Biol. 2014 Jun 9;205(5):707-20. [Content Brief]
[2]. Li D, et al. The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation. Cell Rep. 2019 Apr 9;27(2):343-358.e5. [Content Brief]
[3]. Bem D, et al. Loss-of-function mutations in RAB18 cause Warburg micro syndrome. Am J Hum Genet. 2011 Apr 8;88(4):499-507. [Content Brief]
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