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BRCC36 Antibody (YA2298)

(Synonyms: C6.1A; BRCC36; CXorf53)
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Based on 1 Customer Validation

BRCC36 Antibody (YA2298) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRCC36.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for BRCC36 Antibody (YA2298)
  • BRCC36 Antibody (YA2298) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:100-1:500

Product Details

Description

BRCC36 Antibody (YA2298) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BRCC36.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human BRCC36

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for BRCC36 Antibody (YA2298)
    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), SK-Br-3 (lane 4(20μg), HT-29 (lane 5(20μg) using BRCC36 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    BRCC36 is a Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Acts as a regulator of the NLRP3 inflammasome by mediating deubiquitination of NLRP3, leading to NLRP3 inflammasome assembly. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination. Deubiquitinates HDAC1 and PWWP2B leading to their stabilization[1][2][3][4][5][6][7][8][9][10][11][12].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole

  • Expression


    Tissue_specificity:Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors

  • Isoforms & Post-Translational Modification

    P46736 has 5 isomers: P46736-1: 36072 Da (predicted); P46736-2: 33150 Da (predicted); P46736-3: 33237 Da (predicted); P46736-4: 23458 Da (predicted); P46736-5: 28027 Da (predicted).

  • Subunit

    Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1 (PubMed:20656690, PubMed:24075985).

  • SwissProt ID

    P46736

  • Gene ID
    79184 [NCBI]
  • Synonyms

    C6.1A; BRCC36; CXorf53

  • Research Field

    Cell Biology

BRCC36 Antibody (YA2298) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), SK-Br-3 (lane 4(20μg), HT-29 (lane 5(20μg) using BRCC36 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • BRCC36 Antibody (YA2298) Product Image
    1/16
  • Experimental Validation Results for BRCC36 Antibody (YA2298)
  • BRCC36 Antibody (YA2298) Product Image
  • Experimental Validation Results for BRCC36 Antibody (YA2298)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), SK-Br-3 (lane 4(20μg), HT-29 (lane 5(20μg) using BRCC36 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for BRCC36 Antibody (YA2298)
  • BRCC36 Antibody (YA2298) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), MCF-7 (lane 3(20μg), SK-Br-3 (lane 4(20μg), HT-29 (lane 5(20μg) using BRCC36 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • BRCC36 Antibody (YA2298) Product Image WB