Pan-Lactyl-lysine Antibody (YA6712)

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded human Cervical Cancer‌ tissue using Pan-Lactyl-lysine antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 30 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

MedChemExpress Validation
Immunocytochemistry analysis of Hela cells labeling Pan-Lactyl-lysine with Pan-Lactyl-lysine Antibody (HY-P87019) at 1/1000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Pan-Lactyl-lysine Antibody (HY-P87019) at 1/1000 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Breast Cancer tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

MedchemExpress Validation
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Pan-Lactyl-lysine antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87019, 1:3000 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.