Fibronectin Antibody (YA6107)

製品番号: HY-P86415: データシート SDS COA User Guide for Antibodies
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Fibronectin Antibody (YA6107) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibronectin.

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容量	価格（税別）	在庫状況	数量
10 μL	$105	在庫あり	Estimated Time of Arrival: December 31
50 μL	$276	在庫あり	Estimated Time of Arrival: December 31
100 μL	$432	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Fibronectin Antibody (YA6107) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Fibronectin.

Host

Rabbit

Clonality

Monoclonal

分子量

Predicted band size: 272 kDa;

Observed band size: 285 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P02751

Gene ID

2335 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000-1:5000
WB WB: Western Blot	1:2000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-1:20000

純度	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG,IgG/Kappa

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from HepG2 (lane 2(20μg), NIH/3T3 (lane 3(20μg), Raji (lane 4(20μg), C2C12 (lane 5(20μg) using Fibronectin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Fibronectin Antibody (HY-P86415, 1/4000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Fibronectin Antibody (HY-P86415, 1/4000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human breast tissue using Fibronectin Antibody (HY-P86415, 1/4000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Fibronectin Antibody (HY-P86415, 1/4000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using Fibronectin Antibody (HY-P86415, 1/4000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3593230, PubMed:3900070, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617); Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling; Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling

Subcellular Localization：Secreted, extracellular space, extracellular matrix; Secreted

Expression：
Tissue_specificity:Fibronectin (protein level) is expressed in the internal limiting membrane of the retina and around blood vessels (PubMed: 29777959) . Plasma fibronectin (soluble dimer form) is secreted by hepatocytes. Cellular fibronectin (dimer or cross-linked polymer form) is produced by fibroblasts, epithelial cells, and other cell types and deposited in the extracellular matrix in fibrous form. Urine contains Ugl-Y1, Ugl-Y2, and Ugl-Y3 (PubMed: 17614963) .

Isoforms & Post-Translational Modification：P02751 has 17 isomers: P02751-15: 272320 Da (predicted); P02751-1: 262625 Da (predicted); P02751-2: 71971 Da (predicted); P02751-3: 259216 Da (predicted); P02751-4: 222976 Da (predicted); P02751-5: 243334 Da (predicted); P02751-6: 240509 Da (predicted); P02751-7: 268912 Da (predicted); P02751-8: 252811 Da (predicted); P02751-9: 246688 Da (predicted); P02751-10: 239626 Da (predicted); P02751-11: 262406 Da (predicted); P02751-12: 221292 Da (predicted); P02751-13: 249322 Da (predicted); P02751-14: 249402 Da (predicted); P02751-16: 73683 Da (predicted); P02751-17: 256502 Da (predicted).
Sulfated;It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated;Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers);Phosphorylated by FAM20C in the extracellular medium;Proteolytic processing produces the C-terminal NC1 peptide, anastellin;Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation;Serotonylated on Gln residues by TGM2 in response to hypoxia

Subunit：Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 (By similarity). Interacts with COMP (PubMed:12225811). Interacts (via type III repeats 9-14) with TNFAIP6 (via CUB domain); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1 (PubMed:18042364). Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth (By similarity). Interacts with FST3 and MYOC. Interacts with SVEP1 (By similarity)

Synonyms

FN1; FN; Fibronectin; FN; Cold-insoluble globulin; CIG

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)