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  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. HIF Prolyl Hydroxylases Antibody (YA2975)

HIF Prolyl Hydroxylases Antibody (YA2975)

Cat. No.: HY-P83230
User Guide for Antibodies Technical Support

HIF Prolyl Hydroxylases Antibody (YA2975) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HIF Prolyl Hydroxylases.

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
50 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
100 μL Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks
250 μL   Get quote  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

HIF Prolyl Hydroxylases Antibody (YA2975) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HIF Prolyl Hydroxylases.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 57 kDa;
Observed band size: 43 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human HIF Prolyl Hydroxylases
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex
Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type II membrane protein
Expression：
Tissue_specificity:Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts

Induction:By hypoxia in many cultured cell lines
Isoforms & Post-Translational Modification：Q9NXG6 has 3 isomers: Q9NXG6-1: 56661 Da (predicted); Q9NXG6-2: 47211 Da (predicted); Q9NXG6-3: 63112 Da (predicted).
Glycosylated
Subunit：Homodimer
Database

Entrez Gene: 54681 Human

SwissProt: Q9NXG6 Human

OMIM: 618493 Human

Research Field

Cardiovascular
Synonyms
PH4; PH-4; PHD4; EGLN4; HIDEA; HIFPH4; P4H-TM
Documentation
User Guide for Antibodies (1077 KB)

HIF Prolyl Hydroxylases Antibody (YA2975) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HIF Prolyl Hydroxylases Antibody (YA2975)
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